|
| Status |
Public on Aug 31, 2013 |
| Title |
Expression analysis of Streptococcus mutans UA159 under Hyperosmotic stress environment |
| Organism |
Streptococcus mutans UA159 |
| Experiment type |
Expression profiling by array
|
| Summary |
Oral streptococci metabolize carbohydrate to produce organic acids, which not only decrease the environmental pH, but also increase osmolality of dental plaque fluid due to tooth demineralization and consequent calcium and phosphate accumulation. Despite these unfavorable environmental changes, the bacteria continue to thrive. The aim of this study was to obtain a global view on strategies taken by Streptococcus mutans to deal with physiologically relevant elevated osmolality, and perseveres within a cariogenic dental plaque.
We investigated phenotypic change of S. mutans biofilm upon hyperosmotic challenge. We found that the hyperosmotic condition was able to initiate S. mutans biofilm dispersal by reducing both microbial content and extracellular polysaccharides matrix. We then used whole-genome microarray with quantitative RT-PCR validation to systemically investigate the underlying molecular machineries of this bacterium in response to the hyperosmotic stimuli. Among those identified 40 deferentially regulated genes, down-regulation of gtfB and comC were believed to be responsible for the observed biofilm dispersal. Further analysis of microarray data showed significant up-regulation of genes and pathways involved in carbohydrate metabolism. Specific genes involved in heat shock response and acid tolerance were also upregulated, indicating potential cross-talk between hyperosmotic and other environmental stress.
Hyperosmotic condition induces significant stress response on S. mutans at both phenotypic and transcriptomic levels. In the meantime, it may take full advantage of these environmental stimuli to better fit the fluctuating environments within oral cavity, and thus emerges as numeric-predominant bacterium under cariogenic conditions.
|
| |
|
| Overall design |
A six-chip study using total RNA recovered from mid-logarithmic phase of S. mutans UA159 from three separate cultures of strains submitted for 15 minutes to hyperosmotic stimuli (0.4M NaCl) and three separate cultures of strains kept under no stress condition.
|
| |
|
| Contributor(s) |
Liu C, Niu Y |
| Citation(s) |
24289739 |
| |
| Submission date |
May 22, 2013 |
| Last update date |
Dec 17, 2013 |
| Contact name |
Yulong Niu |
| E-mail(s) |
[email protected]
|
| Organization name |
Max Planck Institute for Plant Breeding Research
|
| Department |
Plant Microbe Interactions
|
| Street address |
Carl-von-Linne-Weg 10
|
| City |
Köln |
| State/province |
Nordrhein-Westfalen |
| ZIP/Postal code |
50829 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL17184 |
Streptococcus mutans UA159 NimbleGen Genechip 72k [gene-level version] |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA204974 |
Less...
More...