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| Status |
Public on Jun 05, 2013 |
| Title |
HOTAIR reprograms gene expression profiling in esophageal squamous cell carcinoma (ESCC) KYSE180 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To investigate the functional role of HOTAIR in esophageal squanmous cell carcinoma (ESCC) cells, RNAi-mediated knockdown of HOTAIR and overexpression of HOTAIR were carried out using KYSE180 cells. Gene expression microarray analysis revealed that a number of reported HOTAIR target genes were regulated by HOTAIR. Moreover, gene ontology analysis revealed enrichment of genes closely related to tumorigenesis, such as cell migration, regulation of cell cycles.
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| Overall design |
For RNAi-mediated knockdown of HOTAIR, two different stealth siRNAs against HOTAIR were generated by Invitrogen, after which a mixture of the two was used for transfection. KYSE180 cells in 6-well plates were transfected with 100 pmol of Stealth siRNA (Invitrogen) or a Stealth RNAi Negative Control Medium GC (Invitrogen) using Lipofectamine2000 (Invitrogen). For HOTAIR overexpression, KYSE180 cells were transfected with LZRS_HOTAIR vector using Polyfect Transfection Reagent (Qiagen). Total RNA was extracted 48 h after transfection.
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| Contributor(s) |
Li X, Han W |
| Citation(s) |
24022190 |
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| Submission date |
Jun 04, 2013 |
| Last update date |
Aug 23, 2018 |
| Contact name |
Weidong Han |
| E-mail(s) |
[email protected]
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| Organization name |
Chinese PLA General Hospital
|
| Department |
School of Life Sciences
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| Lab |
Department of Molecular Biology
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| Street address |
#28 Fuxing Road, HaiDian District
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| City |
Beijing |
| ZIP/Postal code |
100853 |
| Country |
China |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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| Samples (3) |
| GSM1153920 |
KYSE180 control cells |
| GSM1153921 |
KYSE180 cells transfected with HOTAIR vector |
| GSM1153922 |
KYSE180 cells transfected with siRNAs targeting HOTAIR |
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| This SubSeries is part of SuperSeries: |
| GSE47639 |
KYSE180 cells transfected with HOTAIR compared to control cells |
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| Relations |
| BioProject |
PRJNA206999 |
