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| Status |
Public on May 11, 2006 |
| Title |
GDNF-regulated gene expression in cultured mouse spermatogonial stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
GDNF-regulated gene expression was studied in cultures of actively self-renewing spermatogonial stem cells established from 6 day old male mice. GDNF is the essential growth factor regulating mouse spermatogonial stem cell self-renewal. Using a serum-free chemically defined culture system that supports mouse spermatogonial stem cell self-renewal for extended periods of time, GDNF-regulated genes were identified using microarray profiling.
Keywords: GDNF withdrawal and time-course replacement
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| Overall design |
Established cultures of highly enriched self-renewing spermatogonial stem cells were subjeted to withrawal of GDNF and GFRalpha1 for 18-hr followed by replacement of the growth factors for 2, 4, and 8-hr. Gene expression was studied using microarray profiling prior to withdrawal, after withdrawal and at each time-point of GDNF/GFRalpha1 replacement.
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| |
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| Contributor(s) |
Oatley JM, Avarbock MR, Telaranta AI, Fearon DT, Brinster RL |
| Citation(s) |
16740658 |
| |
| Submission date |
May 09, 2006 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Jon Oatley |
| E-mail(s) |
[email protected]
|
| Organization name |
Pennsylvania State University
|
| Department |
Dairy and Animal Science
|
| Street address |
324 Henning
|
| City |
University Park |
| State/province |
PA |
| ZIP/Postal code |
16802 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (15)
|
| GSM108313 |
SSC GDNF/GFRalpha1 18-hr withdrawal Rep1 |
| GSM108314 |
SSC GDNF/GFRalpha1 18-hr withdrawal Rep2 |
| GSM108315 |
SSC GDNF/GFRalpha1 18-hr withdrawal Rep3 |
| GSM108316 |
SSC GDNF/GFRalpha1 2-hr replacement Rep1 |
| GSM108317 |
SSC GDNF/GFRalpha1 2-hr replacement Rep2 |
| GSM108318 |
SSC GDNF/GFRalpha1 2-hr replacement Rep3 |
| GSM108319 |
SSC GDNF/GFRalpha1 4-hr replacement Rep1 |
| GSM108320 |
SSC GDNF/GFRalpha1 4-hr replacement Rep2 |
| GSM108321 |
SSC GDNF/GFRalpha1 4-hr replacement Rep3 |
| GSM108322 |
SSC GDNF/GFRalpha1 8-hr replacement Rep1 |
| GSM108323 |
SSC GDNF/GFRalpha1 8-hr replacement Rep2 |
| GSM108324 |
SSC GDNF/GFRalpha1 8-hr replacement Rep3 |
|
| Relations |
| BioProject |
PRJNA95745 |
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