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| Status |
Public on Jun 27, 2013 |
| Title |
S-adenosylmethionine modifies cocaine-induced DNA methylation and increases locomotor sensitization in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The aim of the study was to investigate whether environmental factors like S-adenosylmethionine (SAM) via affecting epigenome could alter cocaine-induced gene expression and locomotor sensitization in mice.
Using mouse nucleus accumbens (NAc) tissue, whole-genome gene expression profiling revealed that repeated SAM treatment affected a limited number of genes, but significantly modified cocaine-induced gene expression by blunting nonspecifically the cocaine response.
At the gene level, we discovered that SAM modulated cocaine-induced DNA methylation by inhibiting both promoter-associated CpG-island hyper- and hypomethylation in the NAc but not in the reference tissue cerebellum.
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| Overall design |
Total RNA was extracted from the mouse nucleus accumbens (NAc) tissue. Two tissues were combined to a sample, 4 samples per group used. RNA quality and quantity were assessed using the Nano-Drop -1000 spectrophotometer and the Agilent 2100 Bioanalyzer.
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| Contributor(s) |
Anier K, Zharkovsky A, Kalda A |
| Citation(s) |
23684129 |
| |
| Submission date |
Jun 27, 2013 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Kaili Anier |
| E-mail(s) |
[email protected]
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| Organization name |
University of Tartu
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| Street address |
Ravila 19
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| City |
Tartu |
| ZIP/Postal code |
50411 |
| Country |
Estonia |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA209848 |
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