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| Status |
Public on Aug 01, 2006 |
| Title |
Expression profiles of hrpRS- and hrpL- mutants |
| Organism |
Pseudomonas syringae pv. tomato str. DC3000 |
| Experiment type |
Expression profiling by array
|
| Summary |
Pseudomonas syringae pv. tomato DC3000 is a model pathogen infecting tomato and Arabidopsis plants. Genes encoding the type III secretion system and substrate proteins (collectively called TTSS genes) of this bacterium are induced in plants and in minimal medium (MM). The induction of TTSS genes is mediated by HrpL, an alternative sigma factor recognizing the hrp box in the promoter of TTSS genes. The transcription of hrpL is activated by HrpR and HrpS, two homologous DNA binding proteins encoded by the hrpRS operon. Microarray analysis was conducted to evaluate the DC3000 genes regulated by hrpL and hrpRS in MM. The analysis identified a number of novel hrpLactivated genes with a putative TTSS-independent function. Genes regulated by hrpL were mostly regulated by hrpRS in the same manner, but a large number of genes regulated by hrpRS were hrpL-independent, indicating that hrpL represents one branch of the regulatory pathways downstream of hrpRS. The induction of the TTSS genes was associated with down-regulation of the house-keeping genes, indicating that the activation of the TTSS has a cost on the basic cellular activities. The novel genes/pathways identified by the microarray provide new insight into the bacterial functions coordinating with the TTSS.
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| Overall design |
Three samples (biological replicates) of each condition and mutation were analyzed, except for hrpRS-, which has two. 11 Samples analyzed.
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| Contributor(s) |
Lan L, Tang X |
| Citation(s) |
16941902 |
| |
| Submission date |
May 15, 2006 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Xiaoyan Tang |
| E-mail(s) |
[email protected], [email protected]
|
| Phone |
785-532-2334
|
| Fax |
785-532-5692
|
| Organization name |
Kansas State University
|
| Department |
Plant Pathology
|
| Lab |
xiaoyan
|
| Street address |
4702 Throckmorton
|
| City |
manhattan |
| State/province |
KS |
| ZIP/Postal code |
66502 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL3496 |
Pseudomonas syringae pv. tomato str. DC3000 5.6K array |
|
| Samples (11)
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| Relations |
| BioProject |
PRJNA95847 |
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