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| Status |
Public on Jul 20, 2013 |
| Title |
Expression data in wt or mutant Drosophila melanogaster embryos |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
Terminal differentiation of epidermal cells in Drosophila embryos requires the activity of a transcription factor. Svb is necessary and sufficient to induce this process. pri is a regulator of Svb activity, converting it from a repressor into an activator. To characterize the downstream Svb and pri effectors in cell morphogenesis, we performed microarrays in wt, svb -/- (no gene) and pri -/- (svb repressor) mutant conditions.
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| Overall design |
Embryos were selected at a precise 13-15h (after egg laying) stage of development, and manually genotyped for each condition: wt (W samples), svb -/- (R samples) and pri -/- (P samples). 5 independent replicates of 200 embryos are used for each condition. RNA were extracted and hybridized on Affymetrix microarrays.
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| Contributor(s) |
Menoret D |
| Citation(s) |
23972280, 38335295 |
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| Submission date |
Jul 17, 2013 |
| Last update date |
Feb 15, 2024 |
| Contact name |
serge plaza |
| E-mail(s) |
[email protected]
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| Organization name |
toulouseII University
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| Department |
Developmental Biology Center
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| Street address |
118 route de Narbonne
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| City |
Toulouse |
| ZIP/Postal code |
31062 |
| Country |
France |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (15)
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| GSM1193174 |
wt embryos, replicate 4 |
| GSM1193175 |
wt embryos, replicate 5 |
| GSM1193176 |
svb -/- embryos, replicate 1 |
| GSM1193177 |
svb -/- embryos, replicate 2 |
| GSM1193178 |
svb -/- embryos, replicate 3 |
| GSM1193179 |
svb -/- embryos, replicate 4 |
| GSM1193180 |
svb -/- embryos, replicate 5 |
| GSM1193181 |
pri -/- embryos, replicate 1 |
| GSM1193182 |
pri -/- embryos, replicate 2 |
| GSM1193183 |
pri -/- embryos, replicate 3 |
| GSM1193184 |
pri -/- embryos, replicate 4 |
| GSM1193185 |
pri -/- embryos, replicate 5 |
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| Relations |
| BioProject |
PRJNA212792 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE48997_RAW.tar |
27.9 Mb |
(http)(custom) |
TAR (of CEL) |
| GSE48997_differentially_expressed.txt.gz |
11.2 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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