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| Status |
Public on Nov 04, 2013 |
| Title |
Transcriptome analysis of Escherichia coli under various stress conditions |
| Organism |
Escherichia coli |
| Experiment type |
Expression profiling by genome tiling array
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| Summary |
A genome reduced E. coli strain MDS42ΔgalK::Ptet-gfp-kan were applied for the comparative transcriptome analysis. Genome-wide transcriptional changes under high osmotic prresure, high temperature condition and starvation were evaluated.
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| Overall design |
Exponentially growing cells from recoverying stress response were collected for the transcriptome analysis. High osmotic prresure, high temperature condition and starvation were chosen as stressor. In each kind of stress, three level of stresses were applied. Every 3 biological replications were performed.
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| Contributor(s) |
Matsumoto Y, Ying B, Yomo T |
| Citation(s) |
24252326 |
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| Submission date |
Jul 28, 2013 |
| Last update date |
Jan 25, 2015 |
| Contact name |
Bei-Wen Ying |
| E-mail(s) |
[email protected]
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| Phone |
81 29 853 6633
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| Organization name |
University of Tsukuba
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| Street address |
1-1-1 Tennoudai
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| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8572 |
| Country |
Japan |
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| Platforms (1) |
| GPL13336 |
Affymetrix Escherichia coli full sequence array (EcFS) [EcFS_1, EcFS_2, and EcFS_3] |
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| Samples (30)
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| GSM1196963 |
E. coli MDS42, osmotic pressure NaCl 0.20 M, rep 1 |
| GSM1196964 |
E. coli MDS42, osmotic pressure, NaCl 0.20 M, rep 2 |
| GSM1196965 |
E. coli MDS42, osmotic pressure, NaCl 0.20 M, rep 3 |
| GSM1196966 |
E. coli MDS42, osmotic pressure, NaCl 0.45 M, rep 1 |
| GSM1196967 |
E. coli MDS42, osmotic pressure, NaCl 0.45 M, rep 2 |
| GSM1196968 |
E. coli MDS42, osmotic pressure, NaCl 0.45 M, rep 3 |
| GSM1196969 |
E. coli MDS42, osmotic pressure, NaCl 0.55 M, rep 1 |
| GSM1196970 |
E. coli MDS42, osmotic pressure, NaCl 0.55 M, rep 2 |
| GSM1196971 |
E. coli MDS42, osmotic pressure, NaCl 0.55 M, rep 3 |
| GSM1196972 |
E. coli MDS42, temperature change, 40.0 deg C, rep 1 |
| GSM1196973 |
E. coli MDS42, temperature change, 40.0 deg C, rep 2 |
| GSM1196974 |
E. coli MDS42, temperature change, 40.0 deg C, rep 3 |
| GSM1196975 |
E. coli MDS42, temperature change, 41.5 deg C, rep 1 |
| GSM1196976 |
E. coli MDS42, temperature change, 41.5 deg C, rep 2 |
| GSM1196977 |
E. coli MDS42, temperature change, 41.5 deg C, rep 3 |
| GSM1196978 |
E. coli MDS42, temperature change, 41.8 deg C, rep 1 |
| GSM1196979 |
E. coli MDS42, temperature change, 41.8 deg C, rep 2 |
| GSM1196980 |
E. coli MDS42, temperature change, 41.8 deg C, rep 3 |
| GSM1196981 |
E. coli MDS42, starvation, SHX 50 ng/ml, rep 1 |
| GSM1196982 |
E. coli MDS42, starvation, SHX 50 ng/ml, rep 2 |
| GSM1196983 |
E. coli MDS42, starvation, SHX 50 ng/ml, rep 3 |
| GSM1196984 |
E. coli MDS42, starvation, SHX 100 ng/ml, rep 1 |
| GSM1196985 |
E. coli MDS42, starvation, SHX 100 ng/ml, rep 2 |
| GSM1196986 |
E. coli MDS42, starvation, SHX 100 ng/ml, rep 3 |
| GSM1196987 |
E. coli MDS42, starvation, SHX 150 ng/ml, rep 1 |
| GSM1196988 |
E. coli MDS42, starvation, SHX 150 ng/ml, rep 2 |
| GSM1196989 |
E. coli MDS42, starvation, SHX 150 ng/ml, rep 3 |
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| Relations |
| BioProject |
PRJNA213560 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE49296_RAW.tar |
1.4 Gb |
(http)(custom) |
TAR (of CEL) |
| GSE49296_normalized_data_matrix.txt.gz |
603.7 Kb |
(ftp)(http) |
TXT |
| Processed data are available on Series record |
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