|
| Status |
Public on Jun 06, 2006 |
| Title |
Expression data from p63 siRNA in squamous cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
p63, a homologue of the tumor suppressor p53, is critical for the development and maintenance of squamous epithelia. p63 is specifically expressed in the basal layers of stratified epithelial tissues, and is considered to be a specific marker for cells of this type. The role of p63 in tumorigenesis remains poorly defined. Numerous studies have highlighted the oncogenic potential of the predominant p63 isoform, ΔNp63α; however, data suggests that other p63 proteins can act as tumor suppressors or alter the metastatic potential of tumors. ΔNp63α can act as a transcriptional repressor, but the link between the transcriptional functions of p63 and its biological role is still unclear. In this study, we used a loss-of-function approach to investigate the transcriptional programs controlled by p63.
Keywords: cell line panel
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| |
|
| Overall design |
A panel of squamous cell lines, including transformed and non-transformed cells, were transfected with hygromycin selectable vectors encoding p63 siRNA or control, then were selected with Hygromycin B and harvested.
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| |
|
| Contributor(s) |
Barbieri CE, Pietenpol JA |
| Citation(s) |
16885358 |
| |
| Submission date |
Jun 05, 2006 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Jennifer Pietenpol |
| Organization name |
Vanderbilt University Medical Center
|
| Department |
Biochemistry
|
| Lab |
652 PRB
|
| Street address |
23rd Ave South at Pierce
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
| |
|
| Platforms (3) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
| GPL97 |
[HG-U133B] Affymetrix Human Genome U133B Array |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (14)
|
| GSM112317 |
HaCaT RG cells transfected with control vector 1 |
| GSM112318 |
HaCaT RG cells transfected with p63 siRNA 1 |
| GSM112319 |
HaCaT RG cells transfected with control vector 2 |
| GSM112320 |
HaCaT RG cells transfected with p63 siRNA 2 |
| GSM112321 |
SCC-1 cells transfected with control vector 1 |
| GSM112322 |
SCC-1 cells transfected with p63 siRNA 1 |
| GSM112323 |
SCC-1 cells transfected with control vector 2 |
| GSM112324 |
SCC-1 cells transfected with p63 siRNA 2 |
| GSM112325 |
HaCaT cells transfected with control vector |
| GSM112326 |
HaCaT cells transfected with p63 siRNA |
| GSM112327 |
SCC-012 cells transfected with control vector |
| GSM112328 |
SCC-012 cells transfected with p63 siRNA |
| GSM112329 |
SCC-6 cells transfected with control vector |
| GSM112330 |
SCC-6 cells transfected with p63 siRNA |
|
| Relations |
| BioProject |
PRJNA95479 |
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