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| Status |
Public on Jan 01, 2016 |
| Title |
Molecular interaction of bifidobacteria with kefir grains |
| Platform organism |
Bifidobacterium |
| Sample organism |
Bifidobacterium bifidum |
| Experiment type |
Expression profiling by array
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| Summary |
We got insights into the B. bifidum PRL2010 genes whose expression resulted to be affected when bacterial cells were cultivated on kefir and kefiran as the unique carbon source.
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| Overall design |
In order to exploit the transcriptome of PRL2010 grown on kefir and hefiran we performed global transcription profiling using PRL2010 microarrays hybridized with cDNA from the RNA samples of B. bifidum PRL2010 cultivated on these substrates. We isolated mRNA from B. bifidum PRL2010 cells collected from a culture of kefir grains and from PRL2010 cultivated on MRS plus kefiran at upon 12 hours following inoculation. Microarray analysis was performed with an oligonucleotide array based on the B. bifidum PRL2010 genome: a total of 8,130 oligonucleotide probes of 60bp in length were designed on 1707 ORFs using eArray5.0 (Agilent Technologies). 5 Oligos were designed for each gene on a 4x44k Agilent Microarrays(Agilent Technologies, Santa Clara, CA, USA). Replicates were distributed on the chip at random, non-adjacent positions.
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| Contributor(s) |
Ventura M, Turroni F |
| Citation missing |
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| |
| Submission date |
Aug 30, 2013 |
| Last update date |
Jan 01, 2016 |
| Contact name |
Marco Ventura |
| E-mail(s) |
[email protected]
|
| Phone |
+390521905666
|
| Organization name |
University of Parma
|
| Department |
Dept. Life Sciences
|
| Lab |
Probiogenomics Lab.
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| Street address |
Viale delle Scienze 11/A
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| City |
Parma |
| ZIP/Postal code |
43124 |
| Country |
Italy |
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| Platforms (1) |
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| Samples (4)
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| GSM1220345 |
PRL2010 cultivated on kefir as unique carbon source I |
| GSM1220346 |
PRL2010 cultivated on kefir as unique carbon source II |
| GSM1220347 |
PRL2010 cultivated on kefiran as unique carbon source I |
| GSM1220348 |
PRL2010 cultivated on kefiran as unique carbon source II |
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| Relations |
| BioProject |
PRJNA217787 |
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