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| Status |
Public on Sep 11, 2014 |
| Title |
Peg3 mutational effects on reproduction and placenta-specific gene families |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Peg3 (paternally expressed gene 3) is an imprinted gene encoding a DNA-binding protein. This gene plays important roles in controlling fetal growth rates and nurturing behaviors. In the current study, a new mutant mouse model has been generated to further characterize the functions of this DNA-binding protein. Besides known phenotypes, this new mutant model also revealed potential roles of Peg3 in mammalian reproduction. Female heterozygotes produce a much smaller number of mature oocytes than the wild-type littermates, resulting in reduced litter sizes. According to genome-wide expression analyses, several placenta-specific gene families are de-repressed in the brain of Peg3 heterozygous embryos, including prolactin, cathepsin and carcinoembryonic antigen cell adhesion molecule (Ceacam) families. The observed de-repression is more pronounced in females than in males. The de-repression of several members of these gene families is observed even in the adult brain, suggesting potential defects in epigenetic setting of the placenta-specific gene families in the Peg3 mutants. Overall, these results indicate that Peg3 likely controls the transcription of several placenta-specific gene families, and further suggest that this predicted transcriptional control by Peg3 might be mediated through unknown epigenetic mechanisms.
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| Overall design |
Mouse Breeding Two different types of heterozygotes were prepared for breeding experiment: male and female heterozygotes carrying the paternally (+/-p) and maternally (-m/+) transmitted targeted allele. These 4 heterozygotes were bred with their littermates: for Breeding 1, female wild-type littermates x male heterozygotes (+/-P); Breeding 2, female heterozygotes (+/-P) x male wild-type littermates; Breeding 3, female wild-type littermates x male heterozygotes (-m/+); for Breeding 4, female heterozygotes (-m/+) x male wild-type littermates. Genome-wide expression analysis A litter of 14.5-dpc fetuses were harvested from a timed mating between a male heterozygote (-m/+) and wild-type female littermate. Three tissues were obtained from each fetus: embryo head, placenta and amnionic sac. Total 16 samples were used for this series of genome-wide expression analyses: 8 embryo heads and placenta representing two biological replicates of each of the four following combinations: male wild-type, male heterozygote, female wild-type, and female heterozygote.
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| Contributor(s) |
Kim J, Frey WD, He H, Kim H, Ekram MB, Bakshi A, Faisal M, Perera BU, Ye A, Teruyama R |
| Citation(s) |
24391757 |
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| Submission date |
Sep 12, 2013 |
| Last update date |
Aug 08, 2019 |
| Contact name |
Joomyeong Kim |
| E-mail(s) |
[email protected]
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| Phone |
225-578-7692
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| Organization name |
Louisiana State University
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| Department |
Biological Sciences
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| Street address |
202 Life Sciences
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| City |
Baton Rouge |
| State/province |
LA |
| ZIP/Postal code |
70803 |
| Country |
USA |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA219071 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE50818_RAW.tar |
3.1 Mb |
(http)(custom) |
TAR |
| GSE50818_non-normalized.txt.gz |
2.6 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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