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| Status |
Public on Jun 16, 2014 |
| Title |
Transcriptional network analysis in muscle reveals AP-1 as a partner of PGC-1α in the regulation of the hypoxic gene program [microarray: PGC1a_vs_GFP] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Skeletal muscle tissue shows an extraordinary cellular plasticity, but the underlying molecular mechanisms are still poorly understood. Here we use a combination of experimental and computational approaches to unravel the complex transcriptional network of muscle cell plasticity centered on the peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α), a regulatory nexus in endurance training adaptation. By integrating data on genome-wide binding of PGC-1α and gene expression upon PGC-1α over-expression with comprehensive computational prediction of transcription factor binding sites (TFBSs), we uncover a hitherto underestimated number of transcription factor partners involved in mediating PGC-1α action. In particular, principal component analysis of TFBSs at PGC-1α binding regions predicts that, besides the well-known role of the estrogen-related receptor α (ERRα), the activator protein-1 complex (AP-1) plays a major role in regulating the PGC-1α-controlled gene program of hypoxia response. Our findings thus reveal the complex transcriptional network of muscle cell plasticity controlled by PGC-1α.
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| Overall design |
We used microarrays to detect changes in gene expression in C2C12 cells following PGC-1alpha over-expression or GFP (control) over-expression. We used 3 biological replicates for each condition.
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| Contributor(s) |
Baresic M, Salatino S, van Nimwegen E, Handschin C, Kupr B |
| Citation(s) |
24912679 |
| |
| Submission date |
Sep 26, 2013 |
| Last update date |
Jun 17, 2014 |
| Contact name |
Christoph Handschin |
| Organization name |
Biozentrum, University of Basel
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| Street address |
Spitalstrasse 41
|
| City |
Basel |
| ZIP/Postal code |
4056 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL10740 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [probe set (exon) version] |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE51191 |
Transcriptional network analysis in muscle reveals AP-1 as a partner of PGC-1α in the regulation of the hypoxic gene program |
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| Relations |
| BioProject |
PRJNA221569 |
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