|
| Status |
Public on Oct 23, 2013 |
| Title |
Genome-wide incorporation dynamics reveal distinct categories of turnover for the histone variant H3.3 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We developed a system to study the DNA replication-independent turnover nucleosomes containing the histone variant H3.3 in mammalian cells. By measuring the genome-wide incorporation of H3.3 at different time points following epitope-tagged H3.3 expression, we find three categories of H3.3-nucleosome turnover in vivo: rapid turnover, intermediate turnover and, specifically at telomeres, slow turnover. Our data indicate that H3.3-containing nucleosomes at enhancers and promoters undergo a rapid turnover that is associated with active histone modification marks including H3K4me1, H3K4me3, H3K9ac, H3K27ac and the histone variant H2A.Z. The rate of turnover is negatively correlated with H3K27me3 at regulatory regions and with H3K36me3 at gene bodies.
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| |
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| Overall design |
Examination of incorporation dynamics of histone variant H3.3
|
| |
|
| Contributor(s) |
Kraushaar DC, Jin W, Maunakea A, Ha M, Zhao K |
| Citation(s) |
24176123 |
| |
| Submission date |
Oct 21, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Wenfei Jin |
| E-mail(s) |
[email protected]
|
| Organization name |
NHLBI,NIH
|
| Department |
Systems Biology Center
|
| Lab |
Laboratory of Epigenome Biology
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (45)
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| Relations |
| BioProject |
PRJNA223387 |
| SRA |
SRP031784 |
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