|
| Status |
Public on Oct 25, 2013 |
| Title |
Real-time quantitative PCR analysis of hormone-receptor-negative primary breast tumors |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by RT-PCR
|
| Summary |
Total RNA was extracted from FFPE samples of 139 chemotherapy-naïve hormone-receptor-negative breast cancers pooled from three sample sources and assayed for the expression of 21 genes within two previously reported microarray-derived prognostic signatures in 6 RT-PCR multiplexes.
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| |
|
| Overall design |
RT-PCR gene expression profiling. Total RNA was extracted from 10 micron FFPE sections, and expression levels of the 21 genes of interest plus two reference genes were quantified with 6 multiplex RT-PCR TaqMan assays.
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| |
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| Contributor(s) |
Yau C, Sninsky J, Kwok S, Wang A, Degnim A, Ingle JN, Gillett C, Tutt A, Waldman F, Moore D, Esserman L, Benz CC |
| Citation(s) |
24172169 |
| |
| Submission date |
Oct 23, 2013 |
| Last update date |
Jan 24, 2014 |
| Contact name |
Christina Yau |
| E-mail(s) |
[email protected]
|
| Phone |
415-209-2248
|
| Organization name |
Buck Institute of Age Research
|
| Lab |
Benz Lab
|
| Street address |
8001 Redwood Blvd
|
| City |
Novato |
| State/province |
CA |
| ZIP/Postal code |
94945 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17831 |
HRneg prognostic signatures PCR assay panel |
|
| Samples (139)
|
|
| Relations |
| BioProject |
PRJNA224250 |
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