|
| Status |
Public on Sep 02, 2014 |
| Title |
Critical requirement of ERK/MAPK signaling in respiratory tract development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The mammalian genome contains two ERK/MAP kinase kinase genes, Mek1 and Mek2, which encode dual-specificity kinases responsible for ERK/MAP kinase activation. To define the function of ERK/MAPK signaling pathway in lung development, we performed tissue-specific deletions of Mek1 function in a Mek2 null background. Inactivation of both Mek genes in mesenchyme resulted in several phenotypes including giant omphalocele, skeletal defects, pulmonary hypoplasia, abnormal trachea patterning, and death at birth. Microarray analysis with RNA extracted from lungs of E15.5 Dermo1+/Cre, Mek1+/flox;Mek2-/-;Dermo1+/Cre and Mek1flox/flox;Mek2-/-;Dermo1+/Cre embryos was performed to evaluate the molecular impact of the loss of all Mek alleles in mesenchyme on lung development. .
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| Overall design |
Total RNA was isolated from lungs of E15.5 Dermo1+/Cre embryos (control), from E15.5 Mek1+/flox;Mek2- /-;Dermo1+/Cre embryos (experimental) and from E15.5 Mek1flox/flox;Mek2-/-;Dermo1+/Cre embryos (experimental). Four specimens were analyzed per genotype.
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| Contributor(s) |
Jeannotte L, Charron J, Boucherat O |
| Citation(s) |
25100655 |
| |
| Submission date |
Oct 24, 2013 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Eric R Paquet |
| Organization name |
EPFL
|
| Department |
Life sciences
|
| Lab |
Naef lab
|
| Street address |
Station 15 CH - 1015
|
| City |
Lausanne |
| State/province |
Vaud |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
|
| Samples (12)
|
| GSM1250026 |
E15.5_Lung_Dermo1+/Cre_rep4. |
| GSM1250027 |
E15.5_Lung_Mek1+/flox;Mek2-/-;Dermo1+/Cre_rep1. |
| GSM1250028 |
E15.5_Lung_Mek1+/flox;Mek2-/-;Dermo1+/Cre_rep2. |
| GSM1250029 |
E15.5_Lung_Mek1+/flox;Mek2-/-;Dermo1+/Cre_rep3. |
| GSM1250030 |
E15.5_Lung_Mek1+/flox;Mek2-/-;Dermo1+/Cre_rep4. |
| GSM1250031 |
E15.5_Lung_Mek1flox/flox;Mek2-/-;Dermo1+/Cre_rep1. |
| GSM1250032 |
E15.5_Lung_Mek1flox/flox;Mek2-/-;Dermo1+/Cre_rep2. |
| GSM1250033 |
E15.5_Lung_Mek1flox/flox;Mek2-/-;Dermo1+/Cre_rep3. |
| GSM1250034 |
E15.5_Lung_Mek1flox/flox;Mek2-/-;Dermo1+/Cre_rep4. |
|
| Relations |
| BioProject |
PRJNA224160 |
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