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| Status |
Public on Dec 01, 2015 |
| Title |
The Mechanisms Underlying α-Amanitin Resistance in Drosophila melanogaster: A Microarray Analysis |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
The rapid evolution of toxin resistance in animals has important consequences for the ecology of species and our economy. Pesticide resistance in insects has been a subject of intensive study, however, very little is known about how Drosophila species became resistant to natural toxins with ecological relevance, such as α-amanitin that is produced in deadly poisonous mushrooms. Here we performed a microarray study to elucidate the genes, chromosomal loci, molecular functions, biological processes, and cellular components that contribute to the α-amanitin resistance phenotype in Drosophila melanogaster. We suggest that toxin entry blockage through the cuticle, phase I and II detoxification, sequestration in lipid particles, and proteolytic cleavage of α-amanitin contribute in concert to this quantitative trait. We speculate that the resistance to mushroom toxins in Drosophila melanogaster and perhaps in mycophagous Drosophila species has evolved as a cross-resistance to pesticides or other xenobiotic substances.
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| Overall design |
In the study presented here, we had a total of 3 experimental conditions using 2 stocks of Drosophila melanogaster: 5 biological replicates of the Canton-S stock raised on non-toxic food (CS), 5 biological replicates of the α-amanitin resistant Ama-KTT/M/2 stock raised on non-toxic food (KTT), and 6 biological replicates of the the α-amanitin resistant Ama-KTT/M/2 stock raised on food containing the LC-50 concentration of α-amanitin for this stock (KTT-LC50). For each of biological replicate, we extracted total RNA from a pooled 10 larvae in the late third instar.
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| Contributor(s) |
Mitchell CL, Saul MC, Lei L, Wei H, Werner T |
| Citation(s) |
24695618 |
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| Submission date |
Nov 26, 2013 |
| Last update date |
May 04, 2018 |
| Contact name |
Michael C. Saul |
| E-mail(s) |
[email protected]
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| Organization name |
The Jackson Laboratory
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| Lab |
Chesler Lab
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| Street address |
600 Main St
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| City |
Bar Harbor |
| State/province |
ME |
| ZIP/Postal code |
04609 |
| Country |
USA |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (16)
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| GSM1275944 |
Canton-S non-toxic food replicate 4 |
| GSM1275945 |
Canton-S non-toxic food replicate 5 |
| GSM1275946 |
Ama-KTT/M/2 non-toxic food replicate 1 |
| GSM1275947 |
Ama-KTT/M/2 non-toxic food replicate 2 |
| GSM1275948 |
Ama-KTT/M/2 non-toxic food replicate 3 |
| GSM1275949 |
Ama-KTT/M/2 non-toxic food replicate 4 |
| GSM1275950 |
Ama-KTT/M/2 non-toxic food replicate 5 |
| GSM1275951 |
Ama-KTT/M/2 food containing the LC-50 of α-amanitin for Ama-KTT/M/2 replicate 1 |
| GSM1275952 |
Ama-KTT/M/2 food containing the LC-50 of α-amanitin for Ama-KTT/M/2 replicate 2 |
| GSM1275953 |
Ama-KTT/M/2 food containing the LC-50 of α-amanitin for Ama-KTT/M/2 replicate 3 |
| GSM1275954 |
Ama-KTT/M/2 food containing the LC-50 of α-amanitin for Ama-KTT/M/2 replicate 4 |
| GSM1275955 |
Ama-KTT/M/2 food containing the LC-50 of α-amanitin for Ama-KTT/M/2 replicate 5 |
| GSM1275956 |
Ama-KTT/M/2 food containing the LC-50 of α-amanitin for Ama-KTT/M/2 replicate 6 |
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| Relations |
| BioProject |
PRJNA230005 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE52782_RAW.tar |
31.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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