|
| Status |
Public on Apr 21, 2014 |
| Title |
The effect of deficiency of BLT2 receptor on the gene expression in mouse skin during wound healing process |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Examination of gene expression in the skin of C57Black6 mice deficient in BLT2 receptor and control WT before and after skin punching.
|
| |
|
| Overall design |
Total RNA was prepared from punch biopsies (5 mm in diameter) by using the Qiagen RNeasy Fibrous Tissue Kit (Qiagen, Valencia, CA, USA). In case of punched skin, the samples were taken after 2 days of skin punching. RNA from three mice per group was mixed and used for labeling. Probe cDNA was amplified and labeled with an Illumina total prep RNA amplification kit (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol and then used for hybridization with Illumina MouseWG-6 Expression BeadChip arrays. The signals were quantified by using the Illumina BeadStation with BeadArray technology, and the raw data were obtained by using Illumina BeadStudio software.
|
| |
|
| Contributor(s) |
Liu M, Saeki K, Yokomizo T |
| Citation(s) |
24821912 |
| |
| Submission date |
Dec 17, 2013 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Takehiko Yokomizo |
| E-mail(s) |
[email protected]
|
| Phone |
81-3-5802-1030
|
| Organization name |
Juntendo University School of Medicine
|
| Department |
Biochemistry
|
| Street address |
Hongo 2-1-1, Bunkyo-ku
|
| City |
Tokyo |
| ZIP/Postal code |
113-8421 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA231921 |
Less...
More...