|
| Status |
Public on Oct 23, 2014 |
| Title |
ERK signaling regulates opposing functions of JUN family transcription factors in prostate cancer cell migration |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Knockdowns of c-JUN and JUND had opposite effects on PC3 prostate cell migration. We predicted that c-JUN and JUND control the same set of cell migration genes, but in opposite directions. To test this hypothesis, mRNA with expression changes in c-JUN and JUND knockdown PC3 cell lines were compared to mRNA levels in control (luciferase knockdown) PC3 cells by RNA-seq.
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| |
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| Overall design |
mRNA profiles of luciferase knockdown (WT), c-Jun knockdown, and Jun-D knockdown in PC3 cells were generated using deep sequencing, in triplicate, using Illumina HiSeq. Knockdowns were stable shRNA expression from a lentiviral construct selected with puromycin.
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| |
|
| Contributor(s) |
Selvaraj N, Budka JA, Hollenhorst PC |
| Citation(s) |
25332240 |
| |
| Submission date |
Dec 18, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Peter Hollenhorst |
| E-mail(s) |
[email protected]
|
| Organization name |
Indiana University
|
| Department |
Medical Sciences
|
| Street address |
1001 E 3rd St
|
| City |
Bloomington |
| State/province |
Indiana |
| ZIP/Postal code |
47405 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (9)
|
| GSM1294145 |
Luciferase knockdown in PC3 cells using shRNa replicate 1 |
| GSM1294146 |
Luciferase knockdown in PC3 cells using shRNa replicate 2 |
| GSM1294147 |
Luciferase knockdown in PC3 cells using shRNa replicate 3 |
| GSM1294148 |
c-Jun knockdown in PC3 cells using shRNa replicate 1 |
| GSM1294149 |
c-Jun knockdown in PC3 cells using shRNa replicate 2 |
| GSM1294150 |
c-Jun knockdown in PC3 cells using shRNa replicate 3 |
| GSM1294151 |
Jun-D knockdown in PC3 cells using shRNa replicate 1 |
| GSM1294152 |
Jun-D knockdown in PC3 cells using shRNa replicate 2 |
| GSM1294153 |
Jun-D knockdown in PC3 cells using shRNa replicate 3 |
|
| Relations |
| BioProject |
PRJNA232070 |
| SRA |
SRP034601 |
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