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| Status |
Public on Oct 02, 2014 |
| Title |
Gene expression profile of human epidermal keratinocytes in response to IFNgamma, IL-4, IL-6, IL-17A and IL-22 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To understand the role of epidermal keratinocytes in immunopathology of skin diseases with predominant T helper (Th) cell responses, we measured the genome-wide transcriptional profile of human keratinocytes in response to IFNgamma, IL-4, IL-17A or IL-22, major cytokines produced by Th1, Th2, Th17 or Th22 cells, respectively.
IL-6 was also included in the transcriptional profile analysis because a variety of pro-inflammatory stimuli stimulate human keratinocytes to produce IL-6 that has an autocrine or paracrine role in epidermal immunity.
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| Overall design |
We aimed to discover commonly expressed genes in human keratinocytes in response to pro-inflammatory cytokines, which would be associated with common pathophysiological responses in various skin diseases such as skin permeability barrier disruption or epidermal hyperplasia. Normal human keratinocytes (NHKs) were stimulated with IFNγ, IL-4, IL-6, IL-17A and IL-22 for 24 hours and harvested for total RNA extraction and hybridization on Affymetrix microarrays.
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| Contributor(s) |
Noh M |
| Citation(s) |
25168428 |
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| Submission date |
Jan 02, 2014 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Minsoo Noh |
| E-mail(s) |
[email protected]
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| Phone |
82-2-880-2481
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| Organization name |
Seoul National University
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| Department |
Pharmacy
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| Lab |
Systems Pharmacology
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| Street address |
1 Gwanak-ro, Gwanak-gu
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| City |
Seoul |
| ZIP/Postal code |
151-742 |
| Country |
South Korea |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (12)
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| GSM1299978 |
Human primary keratinocytes, vehicle treated, biological replicate 1 |
| GSM1299979 |
Human primary keratinocytes, vehicle treated, biological replicate 2 |
| GSM1299980 |
Human primary keratinocytes, interferon gamma treated, biological replicate 1 |
| GSM1299981 |
Human primary keratinocytes, interferon gamma treated, biological replicate 2 |
| GSM1299982 |
Human primary keratinocytes, interleukin-4 treated, biological replicate 1 |
| GSM1299983 |
Human primary keratinocytes, interleukin-4 treated, biological replicate 2 |
| GSM1299984 |
Human primary keratinocytes, interleukin-17A treated, biological replicate 1 |
| GSM1299985 |
Human primary keratinocytes, interleukin-17A treated, biological replicate 2 |
| GSM1299986 |
Human primary keratinocytes, interleukin-22 treated, biological replicate 1 |
| GSM1299987 |
Human primary keratinocytes, interleukin-22 treated, biological replicate 2 |
| GSM1299988 |
Human primary keratinocytes, interleukin-6 treated, biological replicate 1 |
| GSM1299989 |
Human primary keratinocytes, interleukin-6 treated, biological replicate 2 |
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| Relations |
| BioProject |
PRJNA232810 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE53751_RAW.tar |
56.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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