|
| Status |
Public on Jun 07, 2007 |
| Title |
Clostridium acetobutylicum ATCC 824 60-mer selection |
| Platform organism |
Clostridium acetobutylicum ATCC 824 |
| Sample organism |
Clostridium acetobutylicum |
| Experiment type |
Expression profiling by array
|
| Summary |
Several custom made Agilent arrays were hybridized to select the best 60-mers for the transcriptional profiling of C. acetobutylicum strains.
Keywords: Test of 60-mer performance
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| |
|
| Overall design |
RNA samples were taken from both C. acetobutylicum ATCC and M5 strains at several points during the exponential phase of growth and the samples for each strain were mixed. For the ATCC824 strain the samples were taken at OD (600 nm) = {1.09, 1.8, 2.6, 2.0} whereas for the the M5 strain samples were taken at OD (600 nm) = {0.868, 1.36, 2.40, 3.20, 4.20}. The total amount of labeled material hybridized was 0.75 micrograms.
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| |
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| Contributor(s) |
Paredes CJ, Borden JR, Sillers R, Jones S, Cheng N, Papoutsakis ET |
| Citation(s) |
17526797 |
| |
| Submission date |
Jul 25, 2006 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Eleftherios Terry Papoutsakis |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Delaware
|
| Department |
Chemical Engineering
|
| Street address |
15 Innovation Way
|
| City |
Newark |
| State/province |
DE |
| ZIP/Postal code |
19711 |
| Country |
USA |
| |
|
| Platforms (3) |
| GPL3820 |
NU C. acetobutylicum ATCC 824 13 K whole genome spotted cDNA array |
| GPL4029 |
C. acetobutylicum ATCC824 22K ChIP-on-chip array v1 |
| GPL4030 |
C. acetobutylicum ATCC824 22K Transcriptional array v1 |
|
| Samples (12)
|
| GSM123098 |
Clostridium acetobutylicum 60-mer Selection Test Agilent 60-mer ChIP-on-chip capable array S3 |
| GSM123099 |
Clostridium acetobutylicum 60-mer Selection Test Agilent 60-mer ChIP-on-chip capable array S1 |
| GSM123100 |
Clostridium acetobutylicum 60-mer Selection Test Agilent 60-mer ChIP-on-chip capable array S4 |
| GSM123101 |
Clostridium acetobutylicum 60-mer Selection Test Agilent 60-mer ChIP-on-chip capable array S2 |
| GSM123102 |
Clostridium acetobutylicum 60-mer Selection Test Agilent 60-mer Transcriptional array S3 |
| GSM123103 |
Clostridium acetobutylicum 60-mer Selection Test Agilent 60-mer Transcriptional array S1 |
| GSM123104 |
Clostridium acetobutylicum 60-mer Selection Test Agilent 60-mer Transcriptional array S4 |
| GSM123105 |
Clostridium acetobutylicum 60-mer Selection Test Agilent 60-mer Transcriptional array S2 |
| GSM123116 |
Clostridium_acetobutylicum_60-mer_Selection_Test_cDNA_Spotted_Array_S1 |
| GSM123117 |
Clostridium_acetobutylicum_60-mer_Selection_Test_cDNA_Spotted_Array_S3 |
| GSM123118 |
Clostridium_acetobutylicum_60-mer_Selection_Test_cDNA_Spotted_Array_S2 |
| GSM123119 |
Clostridium_acetobutylicum_60-mer_Selection_Test_cDNA_Spotted_Array_S4 |
|
| Relations |
| BioProject |
PRJNA96293 |
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