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| Status |
Public on Jan 23, 2014 |
| Title |
RNAi mediates post-transcriptional repression of gene expression in fission yeast Schizosaccharomyces pombe [Affymetrix] |
| Platform organism |
Schizosaccharomyces pombe |
| Sample organism |
Schizosaccharomyces pombe 972h- |
| Experiment type |
Expression profiling by genome tiling array
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| Summary |
RNA interference (RNAi) is a gene silencing mechanism conserved from fungi to mammals. Small interfering RNAs are products and mediators of the RNAi pathway and act as specificity factors in recruiting effector complexes. The Schizosaccharomyces pombe genome encodes one of each of the core RNAi proteins, Dicer, Argonaute and RNA-dependent RNA polymerase (dcr1, ago1, rdp1). Even though the function of RNAi in heterochromatin assembly in S. pombe is established, its role in controlling gene expression is elusive. Here, we report the identification of small RNAs mapped anti-sense to protein coding genes in fission yeast. We demonstrate that these genes are up-regulated at the protein level in RNAi mutants, while their mRNA levels are not significantly changed. We show that the repression by RNAi is not a result of heterochromatin formation. Thus, we conclude that RNAi is involved in post-transcriptional gene silencing in S. pombe.
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| Overall design |
Total RNA from wild type, dcr1Δ and rpd1Δ cells; 3 biological replicates for each strain.
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| Contributor(s) |
Smialowska A, Djupedal I, Wang J, Kylsten P, Swoboda P, Ekwall K |
| Citation(s) |
24462781 |
| |
| Submission date |
Jan 17, 2014 |
| Last update date |
May 27, 2014 |
| Contact name |
Agata Smialowska |
| Organization name |
Karolinska Institute
|
| Department |
Biosciences and Nutrition
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| Street address |
Halsovagen 7
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| City |
Huddinge |
| ZIP/Postal code |
141 83 |
| Country |
Sweden |
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| Platforms (1) |
| GPL7715 |
[Sp20b_M] Affymetrix S. pombe Tiling 1.0FR Array |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE54195 |
RNAi mediates post-transcriptional repression of gene expression in fission yeast Schizosaccharomyces pombe |
|
| Relations |
| BioProject |
PRJNA235986 |
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