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| Status |
Public on Dec 31, 2015 |
| Title |
The plasma membrane NADPH oxidase OsRbohA plays a crucial role in developmental regulation and drought-stress response in rice |
| Organism |
Oryza sativa |
| Experiment type |
Expression profiling by array
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| Summary |
Plasma membrane NADPH oxidases (NOXs) are major producers of reactive oxygen species (ROS) in plant cells under normal growth and stress conditions. Rice NOXs have multiple homologs but their functional mechanisms are largely unknown.
We used microarrays to detail the global gene expression profiles in rice wild-type (WT, Dongjin) and a mutant osnox2 which loss the functions of OsNOX2 protein under drought and identified distinct classes of genes between the two type rice plants under both normal growth and drought stressed conditions.
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| Overall design |
The youngest fully expanded leaves from 2.5-month-old WT and osnox2 plants (three replicates each), grown under normal growth (soil moisture, 47.3%) and drought conditions (soil moisture, 8.5%), were used for RNA extraction and hybridization on Affymetrix microarrays. Control: normal growth condition; Drought: drought stress condition.
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| Contributor(s) |
Wang X, Zhou C, Chen K |
| Citation(s) |
26400148 |
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| Submission date |
Jan 28, 2014 |
| Last update date |
Dec 27, 2017 |
| Contact name |
Kunming Chen |
| E-mail(s) |
[email protected]
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| Organization name |
Northwest A&F University
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| Department |
College of Life Sciences
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| Lab |
State Key Laboratory of Crop Stress Biology in Arid Area
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| Street address |
Xinong Road 22#
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| City |
Yangling |
| State/province |
Shaanxi |
| ZIP/Postal code |
712100 |
| Country |
China |
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| Platforms (1) |
| GPL2025 |
[Rice] Affymetrix Rice Genome Array |
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| Samples (12)
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| GSM1315892 |
osnox2 mutant under normal growth condition, biological rep1 |
| GSM1315893 |
osnox2 mutant under normal growth condition, biological rep2 |
| GSM1315894 |
osnox2 mutant under normal growth condition, biological rep3 |
| GSM1315895 |
osnox2 mutant under drought stress condition, biological rep1 |
| GSM1315896 |
osnox2 mutant under drought stress condition, biological rep2 |
| GSM1315897 |
osnox2 mutant under drought stress condition, biological rep3 |
| GSM1315898 |
WT plants under normal growth condition, biological rep1 |
| GSM1315899 |
WT plants under normal growth condition, biological rep2 |
| GSM1315900 |
WT plants under normal growth condition, biological rep3 |
| GSM1315901 |
WT plants under drought stress condition, biological rep1 |
| GSM1315902 |
WT plants under drought stress condition, biological rep2 |
| GSM1315903 |
WT plants under drought stress condition, biological rep3 |
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| Relations |
| BioProject |
PRJNA236586 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE54466_RAW.tar |
49.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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