|
| Status |
Public on Feb 14, 2014 |
| Title |
The effect of GPR30 agonist G-1 on androgen-sensitive and castration-resistant LNCaP xenografts |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
G-1 is an agonist to GPR30. Activation of GPR30 by G-1 inhibited prostate cancer cell growth in LNCaP xenografts regrown after catration of the host (nude mice), but not in the androgen-sensitive LNCaP xenograft grown in an intact host. Results provide insights into the molecular basis of G-1 action in castration-resistant prostate cancer.
|
| |
|
| Overall design |
Male nude mice were injected with LNCaP cells. When the LNCaP tumors reached 150–300 mm3, mice were divided into two groups: intact (androgen-sensitive tumor) and castrated. For the intact group, mice were subcutaneously injected with vehicle alone (95% PBS, 2.5% DMSO, 2.5% ethanol) or G-1 (4 mg/kg/day in vehicle) daily for 16 days. For the castrated group, tumors regressed and then regrew to ~300-400mm3. Mice were treated daily with vehicle or G-1 as described for 16 days. Tumors were harvested for RNA extraction and microarray experiments.
|
| |
|
| Contributor(s) |
Lam H, Chen J, Ouyang B, Medvedovic M, Ho S |
| Citation(s) |
25287069 |
| |
| Submission date |
Feb 13, 2014 |
| Last update date |
Dec 03, 2014 |
| Contact name |
Mario Medvedovic |
| Organization name |
University of Cincinnati
|
| Department |
Department of Environmental Health
|
| Lab |
Laboratory for Statistical Genomics and Systems Biology
|
| Street address |
3223 Eden Av. ML 56
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45267-0056 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL15648 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [HuGene10stv1_Hs_ENTREZG version 13.0.0] |
|
| Samples (16)
|
|
| Relations |
| BioProject |
PRJNA238187 |
Less...
More...