|
| Status |
Public on Apr 10, 2014 |
| Title |
Regulation of S-phase transcription factors by Cdk1 |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
Analysis of gene expression across the cell cycle from wild type cells, and cells expressing alleles of Yox1, Yhp1, Hcm1, and Tos4 that cannot be phosphorylated by Cdk1. Expression of S-phase and M/G1 transcripts are downregulated when phosphorylation of these factors is blocked, demonstrating that Cdk1 promotes expression of late cell cycle genes.
|
| |
|
| Overall design |
These experiments are two-color hybridizations of RNA isolated from synchronized wild type (WT) or phosphomutant (4P) cells, compared to RNA from asyncrhonous wild type cells in mid-log phase. Wild type and mutant cells were synchronized in G1 phase, released into the cell cycle and samples collected at 15 minute intervals. Each time course was carried out in duplicate, the replicate experiment was performed as a dye swap.
|
| |
|
| Contributor(s) |
Landry BD, Benanti JA |
| Citation(s) |
24714560 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R00 GM085013 |
Regulation of Cellular Growth and Division by Ubiquitin-Mediated Proteolysis |
UNIV OF MASSACHUSETTS MED SCHOOL |
Jennifer A Benanti |
|
| |
| Submission date |
Feb 18, 2014 |
| Last update date |
May 18, 2017 |
| Contact name |
Jennifer Benanti |
| E-mail(s) |
[email protected]
|
| Phone |
508-856-1773
|
| Organization name |
University of Massachusetts Medical School
|
| Department |
Molecular, Cell and Cancer Biology
|
| Street address |
364 Plantation St., LRB 525
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16244 |
Agilent-016322 Yeast (V2) Gene Expression 8x15K Microarray (Probe Name version) |
|
| Samples (32)
|
|
| Relations |
| BioProject |
PRJNA238581 |
Less...
More...