|
| Status |
Public on Dec 23, 2014 |
| Title |
Pseudomonas fluorescens transcriptome in flowback water |
| Organism |
Pseudomonas fluorescens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of the study was to examine the influence of flowback water exposure on bacterial survival and biocide tolerance. The transcriptome of P. fluorescens was analyzed using RNA-seq to determine the gene rxpression profile changes occuring upon flowback water exposure. The results indicate that that P. fluorescens induces a well-coordinated genetic response that aids in its survival in flowback water as well as imparts enhanced tolerance against typically used slow acting biocides such as gluteraldehyde but increased susceptibity towards sodium hypochlorite. RNA-seq data demonstrated significant induction of genes involved in osmotic stress, energy production and conversion, membrane integrity, protein transport among others.
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| |
|
| Overall design |
Examination of P. fluorescens transcriptome exposed to flowback water for one hour and compare with PBS exposed cells.
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| |
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| Contributor(s) |
Vikram A, Lipus DC, Bibby KJ |
| Citation(s) |
25279933 |
| |
| Submission date |
Feb 26, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Kyle J Bibby |
| E-mail(s) |
[email protected]
|
| Phone |
412-624-9207
|
| Organization name |
University of Pittsburgh
|
| Department |
Civil and Environmental Engineering
|
| Street address |
3700 O'Hara Street
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15261 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18338 |
Illumina MiSeq (Pseudomonas fluorescens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA239456 |
| SRA |
SRP038984 |
