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| Status |
Public on Jul 09, 2007 |
| Title |
H295R human adrenocortical cells treated with Angiotensin II, Potassium or Forskolin |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
H295R human adrenocortical cells were cultured in H295R complete media containing DMEM:F12 (1:1) supplemented with 2% Ultroser G (Biosepra, Villeneuve-la-Garenne, France), ITS-Plus (Discovery Labware, Bedford, MA) and an antibiotic/antimycotic mixture (Invitrogen, Carlsbad, CA) in 175 cm2 flasks until subconfluente. Media was replaced with 40 ml fresh media containing different agents and cultured for 3 h.
Treatments: Angiotensin II (100 nM), potassium (16 mM) and forskolin (10 uM).
Total RNA extracted with RNAesy Midi Kit (Qiagen) with on-column DNase digestion
Keywords: Agent response
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| Overall design |
Controls, n=2
Angiotensin=1
Potassium=1
Forskolin=1
Same biological sample used for GPL96 and GPL97
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| Contributor(s) |
Romero DG, Rilli S, Plonczynski MW, Yanes LL, Zhou MY, Gomez-Sanchez EP, Gomez-Sanchez CE |
| Citation(s) |
17327493 |
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| Submission date |
Aug 16, 2006 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Damian G. Romero |
| E-mail(s) |
[email protected]
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| Phone |
(601) 984-1523
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| Organization name |
University of Mississippi Medical Center
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| Department |
Biochemistry
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| Street address |
2500 N. State St.
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| City |
Jackson |
| State/province |
MS |
| ZIP/Postal code |
39216 |
| Country |
USA |
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| Platforms (2) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
| GPL97 |
[HG-U133B] Affymetrix Human Genome U133B Array |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA96051 |
| Supplementary data files not provided |
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