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| Status |
Public on Oct 01, 2014 |
| Title |
Bovine Day 15 Extra-embryonic membrane (EEM): Male and Female Control vs. Male and Female CSF2 Methylation |
| Organism |
Bos taurus |
| Experiment type |
Methylation profiling by genome tiling array
|
| Summary |
We aimed to study the effects of CSF2 treatment on the methylome of the Day 15 female and male embryo.
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| Overall design |
Methylomic profiling of bovine extra-embryonic membrane at Day 15 post-insemination produced by IVF. Oocytes were matured and fertilized in vitro using a single Holstein bull. After 5 days in vitro culture, the embryos were either treated with 10 ng/ml bovine recombinant CSF2 or the Control (DPBS/BSA) until Day 7. At Day 7, the embryos were transferred into recipients. At day 15 following insemination, embryos were flushed and collected for total RNA and gDNA extraction. The gender of the embryos were determined by PCR prior to microarray analysis. CSF2 males were compared to Control males while CSF2 females were compared to Control females
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| |
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| Contributor(s) |
Dobbs KB, Gagne D, Fournier E, Sirard M, Robert C, Hansen PJ |
| Citation(s) |
25078682 |
| |
| Submission date |
Mar 13, 2014 |
| Last update date |
Oct 01, 2014 |
| Contact name |
Kyle Dobbs |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Florida
|
| Department |
Animal Sciences
|
| Street address |
2250 SW Shealy Drive
|
| City |
Gainesville |
| State/province |
Florida |
| ZIP/Postal code |
32611 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18384 |
EmbryoGENE DNA Methylation Array Bovine 2x400K v2.0 |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA241182 |
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