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| Status |
Public on Sep 26, 2014 |
| Title |
Ionizing Radiation Induced micro RNA Expression Alterations in Human Peripheral Blood Mononuclear Cells |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Ionizing Radition is known to cause cell damage. Human peripheral blood mononuclear cells have long been used to study radiation induced gene expression profiling. Whithin this study we evaluated gene and microRNA expression alterations of human PBMC irradiated with 60 Gy g-ray. Cells were cultured for 2, 4 and 20h after irradiation before RNA was isolated and Agilent Human microRNA Microarrays was performed.
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| Overall design |
Radiation induced gene expression in human peripheral blood mononuclear cells were measured at 2, 4 and 20 hours after exposure to doses of 60 Gy g-rays. Non radiated cell of each donor and time point were used as contol cells. Four independent experiments were performed at each time (2, 4, or 20 hours and 0 h - only 1 sample per donor) using 4 different donors.
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| Contributor(s) |
Ankermit HJ, Beer L, Mildner M |
| Citation(s) |
25257395, 26567861 |
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| Submission date |
Mar 17, 2014 |
| Last update date |
Jul 29, 2019 |
| Contact name |
Lucian Beer |
| Organization name |
Medical University of Vienna
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| Department |
Department of Surgery
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| Lab |
Christian Doppler Laboratory for the. Diagnosis & Regeneration of Cardiac and Thoracic Diseases
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| Street address |
Währginer Gürtel 18-20
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| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
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| Platforms (1) |
| GPL16770 |
Agilent-031181 Unrestricted_Human_miRNA_V16.0_Microarray (miRBase release 16.0 miRNA ID version) |
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| Samples (28)
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| This SubSeries is part of SuperSeries: |
| GSE55955 |
Ionizing Radiation Induced Gene and micro RNA Expression Alterations in Human Peripheral Blood Mononuclear Cells |
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| Relations |
| BioProject |
PRJNA241428 |
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