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| Status |
Public on Dec 31, 2022 |
| Title |
Comparative gene expression in synergid cells and antipodal cells at anthesis |
| Platform organism |
Oryza sativa |
| Sample organism |
Oryza sativa Japonica Group |
| Experiment type |
Expression profiling by array
|
| Summary |
Synergid cells represent the receptor cell type of angiosperm gametophytes that attract and receive the male pollen tube and are implicated in the transfer of the sperm cells to the target egg and central cells prior to double fertilization. The antipodals represent an accessory cell type that typically degenerates after fertilization; in rice it may initially have a short-lived nutritional role prior to endosperm establishment. We examined the transcriptome of these two cell types using Oryza sativa ssp. japonica (cv Katy) using the Affymetrix 57K rice genome GeneChip to provide an overview of genes activated at receptivity in the synergid and antipodal cells and transcript up- and down-regulated at female gametophyte maturity just prior to fertilization.
This is part of a larger microarray paroject to examine the global program of gene expression during sexual reproduction in Oryza sativa which serves as a model angiosperm in which male and female gametophytic and embryonic cells may be readily isolated and characterized.
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| Overall design |
Two female gametophytic accessory cell types were isolated from living embryo sacs, consisting of isolated synergid cells and antipodal cells collected at floral anthesis.
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| Contributor(s) |
Russell SD, Gou X |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 21, 2014 |
| Last update date |
Dec 31, 2022 |
| Contact name |
Scott D Russell |
| E-mail(s) |
[email protected]
|
| Phone |
405 210-8935
|
| Organization name |
University of Oklahoma
|
| Department |
Dept of Microbiology and Plant Biology
|
| Street address |
770 Van Vleet Oval
|
| City |
Norman |
| State/province |
OK |
| ZIP/Postal code |
73019 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL2025 |
[Rice] Affymetrix Rice Genome Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA242435 |
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