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| Status |
Public on Apr 02, 2015 |
| Title |
High throughput sequencing of B.henselae infected and non-infected I. ricinus salivary glands |
| Organism |
Ixodes ricinus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In order to select genes that are differentially expressed in salivary glands during Ixodes ricinus infection by Bartonella henselae we compare the transcriptome of infected and non-infected salivary glands
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| Overall design |
I. ricinus from a pathogen free colony were infected -or not- with B. henselae at the larval and then the nymphal stage and salivary glands of the resulting females adult were dissected for RNA extraction. 454 sequencing was performed on pooled conditions in order to obtain a reference databank. Infected and non-infected samples were then sequenced separatly with illumina, blaston teh reference datbank and compared.
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| Contributor(s) |
Bonnet SI, Liu XY |
| Citation(s) |
25057911 |
| BioProject |
PRJNA237360 |
| |
| Submission date |
Apr 11, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Sarah Irene Bonnet |
| E-mail(s) |
[email protected]
|
| Phone |
33 1 49 77 46 54
|
| Organization name |
INRA
|
| Department |
Animal Health
|
| Lab |
USC Bartonella tiques
|
| Street address |
23 avenue du general de Gaulle
|
| City |
Maisons Alfort |
| ZIP/Postal code |
94706 |
| Country |
France |
| |
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| Platforms (2) |
| GPL18566 |
454 GS FLX (Ixodes ricinus) |
| GPL18567 |
Illumina Genome Analyzer II (Ixodes ricinus) |
|
| Samples (3) |
| GSM1368016 |
I. ricinus B. henselae infected and non-infected salivary glands |
| GSM1368017 |
I. ricinus salivary glands |
| GSM1368018 |
I. ricinus B. henselae-infected salivary glands |
|
| Relations |
| SRA |
SRP041127 |
