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| Status |
Public on May 01, 2014 |
| Title |
Gene expression profile of DLL4+ and DLL4- Hemato-Endothelial Progenitors (HEPs) subpopulations |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
In hESCs, expression of the Notch ligand DLL4 parallels the emergence of bipotent hematoendothelial progenitors (HEPs) and promotes their hematopoietic differentiation. During differentiation, DLL4 is only expressed in a subpopulation of HEPs. To study the developmental fate of the two subpopulations of HEPs identified by DLL4 expression, we FACS-isolated DLL4high and DLL4low/- HEPs at day 15 of differentiation and performed gene expression analysis using microarrays
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| Overall design |
10^5 DLL4high and DLL4low/- HEPs purified by FACS from H9 and AND1 hEBs at day 15 of hematopoietic differentiation were used for gene expression analysis using Whole Human Genome Oligo Microarray chips (Agilent Technologies).
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| Contributor(s) |
Ayllon V, Menendez P |
| Citation(s) |
25778099 |
| |
| Submission date |
Apr 17, 2014 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Veronica Ayllon |
| E-mail(s) |
[email protected]
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| Organization name |
GENYO. Centre for Genomics and Oncological Research
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| Street address |
Av Ilustracion, 114
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| City |
Granada |
| ZIP/Postal code |
18016 |
| Country |
Spain |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA244867 |
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