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Series GSE57063 Query DataSets for GSE57063
Status Public on Nov 22, 2021
Title Tet enzymes are essential for embryogenesis and completion of embryonic genome activation
Organisms Mus musculus; synthetic construct
Experiment type Expression profiling by high throughput sequencing
Summary Mammalian embryo development begins in transcriptional silence followed by activation of thousands of genes. Reprogramming of DNA methylation is integral to embryogenesis and linked to Tet enzymes; however, their function in early development is not fully understood. Here, we generated combined deficiencies of all three Tet enzymes in mouse oocytes and observed arrest at 2-cell stage with the most severe phenotype linked to Tet2. Gene expression analysis revealed that Tet enzymes are required for completion of embryonic genome activation. Our results demonstrate that Tet enzymes have non-redundant roles and are key components of EGA in mammalian embryos.
 
Overall design RNA-Seq of control-morpholino injected, Tet3-morpholino injected, Tet1-, Tet2-, and Tet3-morpholino injected 2-cell embryos (11 replicates each) as well as alpha-amanitin treated 2-cell embryos (12 replicates) and negative controls (3 replicates)
 
Contributor(s) Arand J, Chiang HR, Snyder MP, Reijo Pera RA, Wossidlo M
Citation(s) 34866320
Submission date Apr 24, 2014
Last update date Feb 03, 2022
Contact name Julia Arand
E-mail(s) [email protected]
Organization name Medical University of Vienna
Street address Schwarzspanierstr. 16
City Vienna
ZIP/Postal code 1090
Country Austria
 
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL15228 Illumina HiSeq 2000 (synthetic construct)
Samples (48)
GSM1373870 control-morpholino injected 2-cell embryo_1
GSM1373871 control-morpholino injected 2-cell embryo_2
GSM1373872 control-morpholino injected 2-cell embryo_3
Relations
BioProject PRJNA245356
SRA SRP041426

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Supplementary file Size Download File type/resource
GSE57063_FPM.txt.gz 887.0 Kb (ftp)(http) TXT
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Processed data are available on Series record
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