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| Status |
Public on Mar 03, 2016 |
| Title |
Role of Fun30 remodelers in retrotransposon regulation [ChIP-chip and expression analysis] |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Expression profiling by genome tiling array
Genome binding/occupancy profiling by genome tiling array
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| Summary |
Retrotransposable elements are genomic parasites that are both key drivers of evolution and the ancestors of retroviruses. Although host cells have developed numerous mechanisms to keep these elements in check, there is also evidence from yeast to mammals that stressed cells sometimes activate transposition. Here we show that the fission yeast tf2 retrotransposons are regulated by alternative transcription start site (TSS) usage. Which TSS is used is determined by nucleosome positioning, which is in turn controlled by the Fun30 chromatin remodelers Fft2 and Fft3. By maintaining a high level of nucleosome occupancy at retrotransposon-flanking Long Terminal Repeat (LTR) elements, Fft2 and Fft3 promote the use of a downstream TSS and the production of RNA incapable of reverse transcription and retrotransposition. More generally, we show that Fft2 and Fft3 are involved in repressing the transcriptional response to stress, of which retrotransposon activation is a part. Finally, we show that in stressed cells retrotransposon TSS usage switches, allowing the production of full-length retrotransposon RNA. We propose that allowing retrotransposon transcription from a nonproductive TSS allows for the rapid activation of these elements in times of stress, while preventing their uncontrolled proliferation in the genome.
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| Overall design |
For ChIP samples, duplicate or triplicate immunoprecipitated samples were compared with mock, input, or WT controls. For expression data duplicate or triplicate mutant cDNA samples were compared with triplicate WT cDNA.
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| Contributor(s) |
Persson J, Steglich B, Smialowska A, Ekwall K |
| Citation(s) |
26902262 |
| |
| Submission date |
Apr 24, 2014 |
| Last update date |
Mar 03, 2016 |
| Contact name |
Babett Steglich |
| Organization name |
Universitätsklinikum Hamburg-Eppendorf
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| Street address |
Martinistrasse 52
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| City |
Hamburg |
| ZIP/Postal code |
20246 |
| Country |
Germany |
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| Platforms (1) |
| GPL7715 |
[Sp20b_M] Affymetrix S. pombe Tiling 1.0FR Array |
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| Samples (7)
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| This SubSeries is part of SuperSeries: |
| GSE57069 |
Role of Fun30 remodelers in retrotransposon regulation |
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| Relations |
| BioProject |
PRJNA245876 |
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