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| Status |
Public on Oct 23, 2006 |
| Title |
Double-tiled DNA microarrays -- proof of principle |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by genome tiling array
|
| Summary |
We have developed a double-tiling method that effectively doubles the number of sequences on a microarray, and with these arrays we confirm that our design can be utilized quickly and easily.
Keywords: testing novel microarray design
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| |
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| Overall design |
GPL4107 is a double-tiled array, and GPL4111 is a conventional array. Both contain the entire yeast genome at approximately 150 and 280 bp resolution, respectively. Our proof-of-principle experiments were done with plasmids; we subsequently performed a more complex experiment (comparing galactose vs glucose-grown yeast) on both the double-tiled arrays and on the conventional array (for use as a standard).
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| |
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| Contributor(s) |
Wheelan SJ, Martinez-Murillo F, Irizarry RA, Boeke JD |
| Citation(s) |
17060914 |
| |
| Submission date |
Sep 01, 2006 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Sarah Wheelan |
| Organization name |
Johns Hopkins University School of Medicine
|
| Department |
Oncology
|
| Street address |
550 N. Broadway
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL4107 |
Hopkins S. Cerevisiae 44k Double Tiling Array v1.0 |
| GPL4111 |
Hopkins S. Cerevisiae 44k TIP-chip v1.0 |
|
| Samples (6)
|
| GSM133545 |
Comparing baseline and galactose-induced yeast expression for comparison with the double-tiled array |
| GSM133546 |
Comparing baseline and galactose-induced yeast expression for comparison with the double-tiled array, replicate |
| GSM133677 |
Demonstration of double tiling with a simple yeast plasmid sample, #1 |
| GSM133678 |
Demonstration of double tiling with a simple yeast plasmid sample, #2 |
| GSM133679 |
Comparing baseline and galactose-induced yeast expression using a double-tiled array |
| GSM133681 |
Comparing baseline and galactose-induced yeast expression using a double-tiled array, replicate |
|
| Relations |
| BioProject |
PRJNA97017 |
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