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| Status |
Public on Sep 22, 2014 |
| Title |
Gene expression profile associated with PTB-mediated transdifferentiation of human fibroblast cells into neurons |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Fibroblasts can be directly converted into neurons in vitro by depletion of nPTB, the reprogramming process induces neuronal gene expression while inhibits fibroblast gene expression. Human fibroblasts lacking CSB can not be converted into neurons, unless CSB gene was introduced. This study characterizes the CSB-dependent gene expression alterations during reprogramming.
Using Nimblegen microarray we identified differentially expressed genes in induced neurons from wild type CSB reconstituted fibroblasts, and we proved that the difference in gene expression after reprogramming was not observed in CS1AN cells.
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| Overall design |
The Nimblegen human 12 x 135K gene expression array was used to define gene expression profiles in PTB-depleted human fibroblasts lacking CSB or expressing reconstituted CSB.
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| Contributor(s) |
Wang Y, Chakravarty P, Svejstrup J |
| Citation(s) |
25249633 |
| |
| Submission date |
May 29, 2014 |
| Last update date |
Oct 01, 2014 |
| Contact name |
Yuming Wang |
| E-mail(s) |
[email protected]
|
| Organization name |
Cancer Research UK
|
| Department |
Mechanisms of Transcription Laboratory
|
| Street address |
South Mimms
|
| City |
London |
| ZIP/Postal code |
EN6 3LD |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL18734 |
NimbleGen Homo sapiens Expression Array [PROBE_ID version; 100718_HG18_opt_expr] |
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| Samples (8)
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| GSM1400348 |
CSB deficient cell line, biological rep 1 |
| GSM1400349 |
CSB deficient cell line, biological rep 2 |
| GSM1400350 |
PTB-depleted CSB deficient cell line, biological rep 1 |
| GSM1400351 |
PTB-depleted CSB deficient cell line, biological rep 2 |
| GSM1400352 |
CSB BAC reconstituted cell line, biological rep 1 |
| GSM1400353 |
CSB BAC reconstituted cell line, biological rep 2 |
| GSM1400354 |
PTB-depleted CSB BAC reconstituted cell line, biological rep 1 |
| GSM1400355 |
PTB-depleted CSB BAC reconstituted cell line, biological rep 2 |
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| This SubSeries is part of SuperSeries: |
| GSE58071 |
Dysregulation of gene expression as the cause of Cockayne syndrome neurologic disease |
|
| Relations |
| BioProject |
PRJNA248788 |
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