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| Status |
Public on Sep 19, 2007 |
| Title |
Effect of oestrogen treatment on growth of human angiomyolipoma xenograft tumours |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
SV7tert AML cells were obtained from ATCC and cultured in Dulbecco's modified essential medium (DMEM), glutamine (4mmol) and 10% foetal bovine serum (FBS). Two million SV7tertAML cells were subcutaneously injected into nude mice either with or without subcutaneous oestrogen pellets (n=4 per group); oestrogen was added using 0.36mg 60 day release oestrogen pellets implanted sub-cutaneously. Mice were housed in pathoflex isolators at 26°C, on 12 hour light / dark cycles. Irradiated RB2 diet and autoclaved water provided ad libertum.
All oestrogen treated mice inoculated with SV7tertAML cells developed subcutaneous tumours which required termination of the animals at six weeks due to tumour load. These primary tumours were removed and fragments placed subcutaneously in further nude mice which went on to develop tumours both in the presence and absence of oestrogen. Mice were weighed weekly and their clinical condition closely monitored by a trained observer. Mice were terminated individually when the tumour cross-sectional area reached 250mm2 or sooner, which has previously been seen to be below the limit of 10% of initial body weight set by the UKCCCR guidelines. Tumours were removed and a portion flash frozen in liquid nitrogen. RNA was extracted from five tumours from oestrogen treated and control animals. Equal quantities of RNA was pooled and quality checked by Agilent Bioanylyser.
Experimenter name = Simon Johnson
Experimenter institute = Queens Medical Centre
Keywords: estrogen treatment, angiomyolipoma xenograft tumors
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| Overall design |
2 samples were used in this experiment
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| |
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| Contributor(s) |
Johnson S, Clements D, Townsend H, Emmerson Z, Schildknecht B |
| Citation(s) |
18988705 |
| |
| Submission date |
Sep 19, 2006 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Simon R. Johnson |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Nottingham
|
| Department |
Therapeutics and Molecular Medicine
|
| Street address |
Queen's Medical Centre
|
| City |
Nottingham |
| ZIP/Postal code |
NG7 2UH |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA97277 |
