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| Status |
Public on Dec 17, 2014 |
| Title |
Cross-feeding by Bifidobacterium breve UCC2003 during Bifidobacterium bifidum PRL2010 growth on a mucin-based medium. |
| Organism |
Bifidobacterium breve UCC2003 |
| Experiment type |
Expression profiling by array
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| Summary |
Bifidobacteria constitute commensal bacteria that commonly inhabit the mammalian gastro intestinal tract. The gut commensal Bifidobacterium breve UCC2003 was previously shown to utilise a variety of plant/diet-derived carbohydrates, including cellodextrin, starch and galactan. In the current study, we investigated the ability of this strain to utilize (parts of) a host-derived source of carbohydrate, namely the mucin glycoprotein. Here, we demonstrate that B. breve UCC2003 exhibits growth properties in a mucin-based medium, but only when in the presence of Bifidobacterium bifidum PRL2010, which is known to metabolize mucin. Based on HPAEC analysis, transcriptome data and insertion mutagenesis, it appears that B. breve UCC2003 sustains this improved survival in co-culture by cross-feeding on a combination of fucose, sialic acid and galactose-containing oligosaccharides.
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| Overall design |
DNA-microarrays containing oligonucleotide primers representing each of the 1864 annotated genes on the genome of B. breve UCC2003 (O'Connell Motherway et al., 2011) were designed by and obtained from Agilent Technologies (Palo Alto, Ca., USA). Methods for cell disruption, RNA isolation, RNA quality control, complementary DNA synthesis and labeling were performed as described previously (Pokusaeva et al., 2009). Labeled cDNA was hybridized using the Agilent Gene Expression hybridization kit (part number 5188-5242) as described in the Agilent Two-Color Microarray-Based Gene Expression Analysis v4.0 manual (G4140-90050). Following hybridization, microarrays were washed in accordance with Agilent’s standard procedures and scanned using an Agilent DNA microarray scanner (model G2565A). Generated scans were converted to data files with Agilent's Feature Extraction software (Version 9.5). DNA-microarray data were processed as previously described (Garcia De La Nava et al., 2003). Differential expression tests were performed with the Cyber-T implementation of a variant of the t-test (Long et al., 2001). A gene was considered differentially expressed when p < 0.001 and an expression ratio of >3 or <0.33 relative to the control.
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| Contributor(s) |
Egan M |
| Citation(s) |
25420416 |
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| Submission date |
Jul 02, 2014 |
| Last update date |
Dec 17, 2014 |
| Contact name |
Mary O'Connell Motherway |
| E-mail(s) |
[email protected]
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| Phone |
00 353 21 490 1364
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| Organization name |
University College Cork
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| Department |
Department of Microbiology and Alimentary Pharmabiotic Centre
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| Street address |
Western Road
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| City |
Cork |
| ZIP/Postal code |
na |
| Country |
Ireland |
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| Platforms (1) |
| GPL8878 |
Bifidobacterium breve UCC2003 Agilent 2x11K format |
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| Samples (1) |
| GSM1424441 |
UCC2003 grown in co-culture with B. bifidum PRL2010 in mucin vs. UCC2003 grown in ribose |
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| Relations |
| BioProject |
PRJNA254107 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE59013_RAW.tar |
3.0 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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