|
| Status |
Public on May 01, 2012 |
| Title |
Transcript profiling of drought responsiveness in two contrasting rice genotypes during vegetative stage stress |
| Platform organism |
Oryza sativa Japonica Group |
| Sample organism |
Oryza sativa |
| Experiment type |
Expression profiling by array
|
| Summary |
Rice (Oryza sativa), the major staple food crop is being cultivated under varying ecosystems ranging from irrigated lowland to rainfed upland environments. Improvement in the rice production under drought prone unfavourable environment depends on the development of drought tolerant genotypes which needs thorough understanding of physiological and molecular events behind the tolerance mechanism. There is considerable genetic variation for drought tolerance mechanism within the cultivated gene pool. To understand the diversity of drought response, two indica rice genotypes namely, i) Apo, an up-land drought tolerant indica veriety from Philippines and ii) IR64, a popular high yielding drought susceptible genotype were selected for this study. We used the 22K rice Oligoarray from Agilent technologies to study the transcript profile in the leaves of the two contrasting rice genotypes under control and drought stressed conditions during vegetative phase.
Keywords: Drought response
|
| |
|
| Overall design |
We used Agilent rice gene chips (G4138A) to investigate the transcript level changes in rice leaf tissues during drought stress. We used two contrasting rice genotypes (IR64 drought susceptible and Apo drought tolerant) differing in their degree of drought tolerance. Plants were grown under green house conditions and drought stress was imposed on 33rd DAS. Leaf sampling was done in both control and drought stressed plants after 6 days of drought stress. Three replications of microarray experiments were carried out by hybridizing the control samples against the drought stressed samples.
|
| |
|
| Contributor(s) |
Satoh K, Mauleon R, Manavalan L, Gan Q, Liu G, Lu T, Jun U, Bruskiewich R, Lafitte R, Kikuchi S, Leung H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Oct 05, 2006 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Raveendran Muthurajan |
| E-mail(s) |
[email protected], [email protected]
|
| Phone |
0063-8-536-2701
|
| Organization name |
International Rice Research Institute
|
| Department |
Entomology and Plant Pathology
|
| Lab |
Molecular Genetics Lab
|
| Street address |
Metro Manila
|
| City |
makati |
| State/province |
manila |
| ZIP/Postal code |
7777 |
| Country |
Philippines |
| |
|
| Platforms (1) |
| GPL892 |
Agilent-012106 Rice Oligo Microarray G4138A (Feature Number version) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA97457 |
Less...
More...