 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 01, 2017 |
| Title |
Zinc acquisition in Pseudomonas aeruginosa |
| Organism |
Pseudomonas aeruginosa PAO1 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
This study addresses the impact of zinc limitation on the opportunistic human pathogen, Pseudomonas aeruginosa. Zinc limitation was assessed in the P. aeruginosa PAO1 strain using an isogenic deletion mutant lacking the periplasmic, zinc solute-binding protein, znuA (PA5498). ZnuA delivers bound zinc to its cognate ABC transporter, ZnuBC, for import into the cytoplasm. Our transcriptional analyses revealed P. aeruginosa to possess a multitude of zinc acquisition mechanisms, each of which were highly up-regulated in the zinc-deficient znuA mutant strain. P. aeruginosa also utilized zinc-independent paralogues of zinc-dependent genes to maintain cellular function under zinc limitation. Together, these data reveal the complex transcriptional response and versatility of P. aeruginosa to zinc depletion.
|
| |
|
| Overall design |
The WT (zinc replete) and znuA mutant strain (zinc limited) were grown in cation-defined media to mid-log phase prior to RNA isolation and analysis.
|
| |
|
| Contributor(s) |
Pederick VG, Eijkelkamp BA, Begg SL, McAllister LJ, Paton JC, McDevitt CA |
| Citation(s) |
26290475 |
| |
| Submission date |
Aug 06, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Bart Eijkelkamp |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Adelaide
|
| Street address |
North Tce
|
| City |
Adelaide |
| State/province |
SA |
| ZIP/Postal code |
5000 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL18782 |
Illumina HiSeq 2500 (Pseudomonas aeruginosa PAO1) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA257669 |
| SRA |
SRP045313 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE60177_PseudomonasDESeq.txt.gz |
286.0 Kb |
(ftp)(http) |
TXT |
| GSE60177_RAW.tar |
800.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
|
|
|
|
 |
