|
| Status |
Public on Aug 22, 2014 |
| Title |
Sequestration of a two-component response regulator by a riboswitch regulated non-coding RNA |
| Organism |
Listeria monocytogenes EGD-e |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Riboswitches are ligand-binding elements contained within the 5’ untranslated regions of bacterial transcripts, which generally regulate expression of downstream open reading frames. Here we show that in Listeria monocytogenes, a Vitamin B12-binding (B12) riboswitch controls expression of a non-coding regulatory RNA, Rli55. Rli55 in turn controls expression of the eut genes, which enable ethanolamine utilization and require B12 as a cofactor. Defects in ethanolamine utilization, or in its regulation by Rli55, significantly attenuate Listeria virulence in mice. Rli55 functions by sequestering the two-component response regulator, EutV via a EutV binding-site contained within the RNA. Thus Rli55 is a riboswitch-regulated member of the small group of regulatory RNAs that function by sequestering a protein and reveals a unique mechanism of signal integration in bacterial gene regulation.
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| |
|
| Overall design |
RNA-Seq and CLIP-Seq to investigate the sequestration of EutV by rli55
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| |
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| Contributor(s) |
Mellin JR, Koutero M, Dar D, Nahori M, Sorek R, Cossart P |
| Citation(s) |
25146292 |
| |
| Submission date |
Aug 12, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Jeffrey Mellin |
| E-mail(s) |
[email protected]
|
| Organization name |
Institut Pasteur
|
| Street address |
25 rue Docteur Roux
|
| City |
Paris |
| ZIP/Postal code |
75015 |
| Country |
France |
| |
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| Platforms (2) |
| GPL19075 |
Illumina HiSeq 2500 (Listeria monocytogenes EGD-e) |
| GPL19076 |
Illumina MiSeq (Listeria monocytogenes EGD-e) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA258096 |
| SRA |
SRP045450 |
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