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| Status |
Public on Jun 05, 2015 |
| Title |
Cooperative target mRNA destabilization and translation inhibition by miR-58 microRNA family in C. elegans [RNA-Seq] |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In animals, microRNAs frequently form families with related sequences. The functional relevance of miRNA families and the relative contribution of family members to target repression have remained, however, largely unexplored. Here, we used the C. elegans miR-58 miRNA family, comprised primarily of four highly abundant members: miR-58.1, miR-80, miR-81 and miR-82, as a model to investigate the redundancy of miRNA family members and their impact on target expression in an in vivo setting.
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| Overall design |
RNA was extracted from different miR-58 family mutants (mir-58.1, mir-80; mir-58.1 and mir-80; mir-58.1; mir-81-82) and wild-type Bristol C. elegans strain at late L4 stage and submitted to transcriptome sequencing with Illumina HiSeq2000. The goal was to compare miR-58 target RNA expression and system-wide perturbations across various samples.
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| Contributor(s) |
Subasic D, Bruemmer A |
| Citation(s) |
26232411 |
| |
| Submission date |
Aug 14, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Deni Subasic |
| Organization name |
University of Zurich
|
| Department |
Institute of Molecular Life Sciences
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| Lab |
Michael Hengartner
|
| Street address |
Wintethurerstr. 190
|
| City |
Zurich |
| ZIP/Postal code |
8057 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL13657 |
Illumina HiSeq 2000 (Caenorhabditis elegans) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE60421 |
Cooperative target mRNA destabilization and translation inhibition by miR-58 microRNA family in C. elegans |
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| Relations |
| BioProject |
PRJNA258285 |
| SRA |
SRP045499 |
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