|
| Status |
Public on Oct 18, 2007 |
| Title |
1.0 mM As(III) stressed yap1 deletion mutant vs 1.0 mM As(III) stressed wild-type after 1 hour |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
volved enabling cells to evade toxicity and acquire tolerance. Herein, we
explored how Saccharomyces cerevisiae (budding yeast) respond to trivalent
arsenic (arsenite) by quantitative and kinetic transcriptome, proteome and
sulfur metabolite profiling. Arsenite exposure affected transcription of
genes encoding functions related to protein biosynthesis, arsenic
detoxification, oxidative stress defense, redox maintenance and
proteolytic activity. Importantly, enzymes involved in sulfate assimilation
and glutathione biosynthesis were induced at both gene and protein levels.
Kinetic metabolic profiling evidenced a significant increase in the pools of
sulfur metabolites as well as elevated glutathione levels. Moreover, the
flux in the sulfur assimilation pathway as well as the glutathione synthesis
rate strongly increased with a concomitant reduction of sulfur incorporation
into proteins. By combining comparative genomics and molecular analyses, we
pin-pointed transcription factors that mediate thecore of the
transcriptional response to arsenite. Taken together, our data reveals that
arsenite-exposed cells channel a large part of assimilated sulfur into
glutathione biosynthesis and we provide evidence that the transcriptional
regulators Yap1p and Met4p control this response in concert.
Keywords: stress
|
| |
|
| Overall design |
Direct comparisons
|
| |
|
| Contributor(s) |
Thorsen M, Kristiansson E, Tamas M, Nerman O |
| Citation(s) |
17327492 |
| |
| Submission date |
Oct 18, 2006 |
| Last update date |
Mar 17, 2012 |
| Contact name |
Erik Kristiansson |
| E-mail(s) |
[email protected]
|
| Organization name |
Chalmers University of Technology
|
| Department |
Department of Mathematical Sciences
|
| Street address |
Chalmers Tvärgata 3
|
| City |
Göteborg |
| ZIP/Postal code |
412 96 |
| Country |
Sweden |
| |
|
| Platforms (1) |
| GPL4462 |
Saccharomyces cerevisiae 6.4K array (Y6.4K), version 4 |
|
| Samples (3) |
| GSM140787 |
1.0 mM As(III) stressed YAP1 mutant vs 1.0 mM As(III) stressed wild-type after 1 hour replicate 1 |
| GSM140788 |
1.0 mM As(III) stressed YAP1 mutant vs 1.0 mM As(III) stressed wild-type after 1 hour replicate 2 |
| GSM140789 |
1.0 mM As(III) stressed YAP1 mutant vs 1.0 mM As(III) stressed wild-type after 1 hour replicate 3 |
|
| This SubSeries is part of SuperSeries: |
| GSE6129 |
Arsenate stress response: Saccharomyces cerevisiae |
|
| Relations |
| BioProject |
PRJNA104445 |
