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| Status |
Public on Aug 29, 2014 |
| Title |
gene expression profiles in KRAS mutant A549 cells after BI-2536 and fasudil combination treatment |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To identify the potential mechanisms of enhanced activity of combined inhibition of PLK1 and ROCK, we conducted a microarray analysis on gene expression profiles in KRAS mutant A549 cells.
We used Affymetrix GeneChip PrimeView Human Gene Expression array to detail gene expression after indicated treatments and identified the molecular mechanism during this process to facilitate further studies.
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| Overall design |
A549 cells were treated with vehicle control, BI-2536 (4 nM), fasudil (20 µM), or the combination of BI-2536 and fasudil for 6 h. Samples were obtained from three independent biological replicates.RNA from each sample was labeled and hybridized using Affymetrix GeneChip PrimeView Human Gene Expression array.
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| Contributor(s) |
Wang J, Hu K, Liu M, Pang X |
| Citation(s) |
27193833 |
| |
| Submission date |
Aug 28, 2014 |
| Last update date |
Jul 24, 2019 |
| Contact name |
Xiufeng Pang |
| E-mail(s) |
[email protected]
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| Phone |
+86-21-24206942
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| Organization name |
East China Normal University
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| Department |
School of Life Sciences
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| Lab |
Institute of Biomedical Sciences
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| Street address |
500 Dongchuan Road
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| City |
Shanghai |
| ZIP/Postal code |
200241 |
| Country |
China |
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| Platforms (1) |
| GPL16043 |
GeneChip® PrimeView™ Human Gene Expression Array (with External spike-in RNAs) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA259746 |
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