|
| Status |
Public on Sep 13, 2014 |
| Title |
Ribosome profiling upon inhibition of eIF4A |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Ribosome profiling of MDA-MB-231 cells treated with Silvestrol to monitor transcriptome wide, eIF4A-dependent changes in translation efficiency
|
| |
|
| Overall design |
Translation efficiency (TE) of mRNAs dervied from ribosome footprints was monitored in the presence or absence of 25 nM Silvestrol, an inhibitor of eukaryotic translation initiation factor 4A (eIF4A). Transcripts with reduced TE in the presence of Silvestrol were compare to transcripts with reduced TE in the presence of INK128, a catalytic mTOR inhbitor.
|
| |
|
| Contributor(s) |
Rubio CA, Weisburd B, Holderfield M, Arias C, Fang E, DeRisi JL, Fanidi A |
| Citation(s) |
25273840 |
| |
| Submission date |
Sep 12, 2014 |
| Last update date |
Jan 28, 2020 |
| Contact name |
Claudia Ani Rubio |
| E-mail(s) |
[email protected]
|
| Organization name |
Novartis Institutes for Biomedical Research
|
| Department |
Oncology
|
| Street address |
5300 Chiron Way
|
| City |
Emeryville |
| State/province |
CA |
| ZIP/Postal code |
94608 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (13)
|
|
| Relations |
| BioProject |
PRJNA260909 |
| SRA |
SRP047065 |
Less...
More...