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| Status |
Public on Feb 10, 2017 |
| Title |
Insights into the Staphylococcus aureus-host interface: global changes in host and pathogen gene expression in a rabbit skin infection model |
| Platform organism |
Staphylococcus aureus subsp. aureus USA300 |
| Sample organism |
Staphylococcus aureus |
| Experiment type |
Expression profiling by array
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| Summary |
Staphylococcus aureus is an important cause of human skin and soft tissue infections (SSTIs) globally. Notably, 80% of all SSTIs are caused by S. aureus, of which ~63% are abscesses and/or cellulitis. Although progress has been made, our knowledge of the host and pathogen factors that contribute to the pathogenesis of SSTIs is incomplete. To provide a more comprehensive view of this process, we monitored changes in the S. aureus transcriptome and selected host proinflammatory molecules during abscess formation and resolution in a rabbit skin infection model. Within the first 24 h, S. aureus transcripts involved in DNA repair, metabolite transport, and metabolism were up regulated, suggesting an increase in the machinery encoding molecules involved in replication and cell division. There was also increased expression of genes encoding virulence factors, namely secreted toxins and fibronectin and/or fibrinogen-binding proteins. Of the host genes tested, we found that transcripts encoding host IL-8, IL1β, oncostatin M-like, CCR1, CXCR1 (IL8RA), CCL4 (MIP-1β) and CCL3 (MIP1α)-like proteins were among the most highly up-regulated transcripts during S. aureus abscess formation. Our findings provide additional insight into the pathogenesis of S. aureus SSTIs, including a temporal component of the host response. These results serve as a springboard for future studies directed to better understand how/why mild or moderate SSTIs progress to invasive disease.
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| Overall design |
Staphylococcus aureus inoculum vs. day 1 of abscess
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| Contributor(s) |
Malachowa N, Kobayashi SD, Sturdevant DE, Scott DP, DeLeo FR |
| Citation(s) |
25719526 |
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| Submission date |
Sep 23, 2014 |
| Last update date |
Feb 10, 2017 |
| Contact name |
Dan Sturdevant |
| E-mail(s) |
[email protected]
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| Phone |
4063639248
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| Organization name |
NIH
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| Department |
NIAID
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| Lab |
RTS
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| Street address |
903 S 4th street
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| City |
Hamilton |
| State/province |
MT |
| ZIP/Postal code |
59840 |
| Country |
USA |
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| Platforms (1) |
| GPL8069 |
Affymetrix RML Custom Pathogenic chip 7 |
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| Samples (7)
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| GSM1510542 |
Staphylococcus aureus day 1 abscess left rep 1 |
| GSM1510543 |
Staphylococcus aureus day 1 abscess right rep 2 |
| GSM1510544 |
Staphylococcus aureus day 1 abscess left rep 3 |
| GSM1510545 |
Staphylococcus aureus day 1 abscess right rep 4 |
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| Relations |
| BioProject |
PRJNA261800 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE61669_RAW.tar |
8.0 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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