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| Status |
Public on Nov 10, 2006 |
| Title |
Gene expression profiling: breast cancer formation in WAP-SVT/t transgenic animals |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Microarray studies revealed that as a first hit, SV40 T/t-antigen causes deregulation of 462 genes in mammary gland cells (ME-cells) of WAP-SVT/t transgenic animals. The majority of deregulated genes are cell-proliferation specific and Rb-E2F dependent, causing ME-cell proliferation and gland hyperplasia but not breast cancer formation. In the breast tumor cells, a further 207 genes are differentially expressed, most of them belonging to the cell communication category. In tissue culture, breast tumor cells frequently switch off WAP-SVT/t transgene expression and regain the morphology and growth characteristics of normal-ME-cells, although the tumor-revertant cells are aneuploid and only 114 genes regain the expression level of normal-ME-cells. The profile of retransformants shows that only 38 deregulated genes appear to be tumor-relevant and that none of them is considered to be a typical breast cancer gene.
Keywords: Tumorigenesis, Breast cancer, SV40 T/t antigen
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| Overall design |
We have analyzed nine mammary gland tissue segments from three normal NMRI, three WAP-SVT/t mice on the first day of lactation and from three WAP-SVT/t breast cancers (583-tumor 1, 585-tumor 2, 597-tumor 3) as well as three distinct breast cancer derived cell lines (ME-A cells, revertant-ME-B cells and ME-B-T/t cells).
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| Web link |
http://www.charite.de/molbiol/bioinf/
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| Contributor(s) |
Klein A, Guhl E, Zollinger R, Tzeng Y, Wessel R, Hummel M, Graessmann M, Graessmann A |
| Citation(s) |
15662539 |
| |
| Submission date |
Nov 08, 2006 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Andreas Klein |
| E-mail(s) |
[email protected]
|
| Phone |
+49 30 450 528087
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| Fax |
+49 30 450 52945
|
| URL |
http://www.charite.de/molbiol/bioinf/tumbiol/
|
| Organization name |
Charité (CCO)
|
| Department |
Institute of Biochemistry
|
| Street address |
Charitéplatz 1
|
| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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| Samples (12)
|
| GSM143330 |
NOR LAK sample III: NMRI mouse on the first day of lactation |
| GSM143697 |
NOR LAK sample I: NMRI mouse on the first day of lactation |
| GSM143891 |
NOR LAK sample II: NMRI mouse on the first day of lactation |
| GSM143903 |
WAP-SVT/t-LAK sample I: WAP-SVT/t mouse on the first day of lactation |
| GSM143904 |
WAP-SVT/t-LAK sample II: WAP-SVT/t mouse on the first day of lactation |
| GSM143906 |
WAP-SVT/t-LAK sample III: WAP-SVT/t mouse on the first day of lactation |
| GSM143908 |
583 tumor 1: WAP-SVT/t breast cancer |
| GSM143909 |
585pos1 tumor 2: WAP-SVT/t breast cancer |
| GSM143911 |
597pos7 tumor 3: WAP-SVT/t breast cancer |
| GSM143913 |
ME-A cells: breast cancer cell line |
| GSM143915 |
Revertant ME-B cells: breast cancer cell line |
| GSM143916 |
Retransformed ME-B-T/t cells: breast cancer cell line |
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| Relations |
| BioProject |
PRJNA97521 |
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