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| Status |
Public on Dec 03, 2014 |
| Title |
Expression data from individual MEF2A isoform knockdown in C2C12 myotubes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Differentiation of muscle tissue is regulated by a complex network of transcription factors. The MEF2 family of transcription factors are important players in muscle development and differentiation.
We knocked down expression of MEF2 isoforms in a cell culture model of skeletal muscle differentiation and assessed global expression pattern changes between MEF2 knockdowns, and with a negative control.
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| Overall design |
C2C12 myoblasts were transduced with shRNA viral vectors to knockdown expression of individual MEF2 proteins. Cells were then induced to differentiate and were harvested 3 days post-differentiation for Affymetrix global gene expression analysis. Each array was pooled RNA from two separate transductions, and each treatment group was analyzed in triplicate.
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| Contributor(s) |
Naya FJ, Estrella NL, Desjardins CA, Nocco SE, Clark AL, Maksimenko Y |
| Citation(s) |
25416778 |
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| Submission date |
Dec 02, 2014 |
| Last update date |
Jun 25, 2019 |
| Contact name |
Boston University Microarray and Sequencing Resource |
| E-mail(s) |
[email protected]
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| Organization name |
Boston University
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| Department |
Microarray and Sequencing Resource
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| Street address |
72 East Concord Street, E631
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02118 |
| Country |
USA |
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| Platforms (1) |
| GPL19485 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [CDF: MoGene10stv1_Mm_ENTREZG_16.0.0] |
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| Samples (15)
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| GSM1557594 |
Mef2a knockdown, replicate 1 |
| GSM1557595 |
Mef2a knockdown, replicate 2 |
| GSM1557596 |
Mef2a knockdown, replicate 3 |
| GSM1557597 |
Mef2b knockdown, replicate 1 |
| GSM1557598 |
Mef2b knockdown, replicate 2 |
| GSM1557599 |
Mef2b knockdown, replicate 3 |
| GSM1557600 |
Mef2c knockdown, replicate 1 |
| GSM1557601 |
Mef2c knockdown, replicate 2 |
| GSM1557602 |
Mef2c knockdown, replicate 3 |
| GSM1557603 |
Mef2d knockdown, replicate 1 |
| GSM1557604 |
Mef2d knockdown, replicate 2 |
| GSM1557605 |
Mef2d knockdown, replicate 3 |
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| Relations |
| BioProject |
PRJNA269087 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE63798_RAW.tar |
81.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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