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| Status |
Public on Dec 04, 2014 |
| Title |
Vaccine-Elicited CD4 T Cells Induce Immunopathology Following Chronic LCMV Infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
CD4 T cells promote innate and adaptive immune responses, but how vaccine-elicited CD4 T cells contribute to immune protection remains unclear.
Here we evaluated whether induction of virus-specific CD4 T cells by vaccination would protect mice against infection with chronic lymphocytic choriomeningitis virus (LCMV).
Immunization with vaccines that selectively induced CD4 T cell responses resulted in catastrophic inflammation and mortality following challenge with a persistent form of LCMV.
Immunopathology required antigen-specific CD4 T cells and was associated with a cytokine storm, generalized inflammation, and multi-organ system failure. Virus-specific CD8 T cells or antibodies abrogated the pathology.
These data demonstrate that vaccine-elicited CD4 T cells in the absence of effective antiviral immune responses can trigger lethal immunopathology.
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| Overall design |
Splenic GP66-specific CD4 T cells from mice immunized with either a LMwt vaccine (sham) or LMgp61 vaccine (CD4 vaccine) were purified by FACS on day 8 post-infection with LCMV clone 13
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| Contributor(s) |
Penaloza-MacMaster P, Provine NM, Barouch DH |
| Citation(s) |
25593185 |
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| Submission date |
Dec 03, 2014 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Nicholas Provine |
| E-mail(s) |
[email protected]
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| Organization name |
University of Oxford
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| Department |
NDM - Experimental Medicine
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| Lab |
Paul Klenerman
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| Street address |
John Radcliffe Hospital
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| City |
Oxford |
| State/province |
Oxfordshire |
| ZIP/Postal code |
OX3 9DU |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA269164 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE63825_RAW.tar |
29.0 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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