 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Feb 10, 2017 |
| Title |
RNA sequencing of CACO-2 cells incubated with bifidobacteria grown on human milk oligosaccharides. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Background: Breastfed human infants are predominantly colonized by bifidobacteria that thrive on human milk oligosaccharides (HMO). The two most predominant species of bifidobacteria in infant feces are Bifidobacterium breve (B. breve) and Bifidobacterium longum subsp. infantis (B. infantis), both avid HMO-consumer strains. Our laboratory has previously shown that B. infantis, when grown on HMO, increase adhesion to intestinal cells and increase the expression of the anti-inflammatory cytokine interleukin-10. The purpose of the current study was to investigate the effects of carbon source—glucose, lactose, or HMO—on the ability of B. breve and B. infantis to adhere to and affect the transcription of intestinal epithelial cells on a genome-wide basis.
Results: HMO-grown B. infantis had higher percent binding to Caco-2 cell monolayers compared to B. infantis grown on glucose or lactose. B. breve had low adhesive ability regardless of carbon source. Despite differential binding ability, both HMO-grown strains significantly differentially affected the Caco-2 transcriptome compared to their glucose or lactose grown controls. HMO-grown B. breve and B. infantis both down-regulated genes in Caco-2 cells associated with chemokine activity.
Conclusion: The choice of carbon source affects the interaction of bifidobacteria with intestinal epithelial cells. HMO-grown bifidobacteria reduce markers of inflammation, compared to glucose or lactose-grown bifidobacteria. In the future, the design of preventative or therapeutic probiotic supplements may need to include appropriately chosen prebiotics.
|
| |
|
| Overall design |
CACO-2 cells incubated with Bifidobacterium longum subsp. infantis grown on (1) glucose, (2) lactose, or (3) human milk oligosaccharides. All experiments were run in triplicate.
|
| |
|
| Contributor(s) |
Mills DA, Wickramasinghe S, Lemay DG, Pacheco AR |
| Citation(s) |
26303932 |
| |
| Submission date |
Dec 08, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Danielle G Lemay |
| E-mail(s) |
[email protected]
|
| Organization name |
University of California Davis
|
| Department |
Genome Center
|
| Street address |
451 Health Sciences Dr
|
| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (9)
|
| GSM1561121 |
CACO-2 cells incubated with lactose-grown B. infantis, replicate 1 |
| GSM1561122 |
CACO-2 cells incubated with lactose-grown B. infantis, replicate 2 |
| GSM1561123 |
CACO-2 cells incubated with lactose-grown B. infantis, replicate 3 |
| GSM1561124 |
CACO-2 cells incubated with glucose-grown B. infantis, replicate 1 |
| GSM1561125 |
CACO-2 cells incubated with glucose-grown B. infantis, replicate 2 |
| GSM1561126 |
CACO-2 cells incubated with glucose-grown B. infantis, replicate 3 |
| GSM1561127 |
CACO-2 cells incubated with HMO-grown B. infantis, replicate 1 |
| GSM1561128 |
CACO-2 cells incubated with HMO-grown B. infantis, replicate 2 |
| GSM1561129 |
CACO-2 cells incubated with HMO-grown B. infantis, replicate 3 |
|
| Relations |
| BioProject |
PRJNA269586 |
| SRA |
SRP050900 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE63950_Infantis_GLUvsHMOvsLAC.xlsx |
1.2 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...