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| Status |
Public on Dec 01, 2006 |
| Title |
Cultured A549 lung cancer cells treated with actinomycin D and sapphyrin PCI-2050 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The goal of this project was to characterize changes in gene expression in response to the anti-cancer agent sapphyrin PCI-2050. Cultured A549 human lung cancer cells were treated with sapphyrin PCI-2050 or actinomycin D, a known transcripitonal inhibitor. The gene expression profiles of drug-treated and control A549 cultures were determined using Human Genome U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA). Further details are provided in our published manuscript: <http://www.molecular-cancer.com/content/6/1/9>.
Keywords: responses to treatments with anti-cancer agents
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| |
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| Overall design |
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eight days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, sapphyrin PCI-2050 (1.25 or 2.5 μM final concentration), actinomycin D (5 μg/mL final concentration), or control (5% mannitol) solution was added to the cultures. Each experiment was performed in triplicate. After incubation, all cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated and subjected to analysis on Human Genome U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA).
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| |
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| Contributor(s) |
Hacia JG, Magda D |
| Citation(s) |
17233922 |
| |
| Submission date |
Nov 29, 2006 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Joseph Gerard Hacia |
| E-mail(s) |
[email protected]
|
| Phone |
323-442-3030
|
| Organization name |
University of Southern California
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Hacia Lab
|
| Street address |
2250 Alcazar Street, IGM 261
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90089 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (12)
|
| GSM147099 |
Actinomycin D treated A549 human lung cancer cell cultures replicate A |
| GSM147363 |
Actinomycin D treated A549 human lung cancer cell cultures replicate B |
| GSM147364 |
Actinomycin D treated A549 human lung cancer cell cultures replicate C |
| GSM147365 |
Mannitol control treated A549 human lung cancer cell cultures replicate A |
| GSM147366 |
Mannitol control treated A549 human lung cancer cell cultures replicate B |
| GSM147367 |
Mannitol control treated A549 human lung cancer cell cultures replicate C |
| GSM147368 |
1.25uM sapphyrin PCI-2050 treated A549 human lung cancer cell cultures replicate A |
| GSM147369 |
1.25uM sapphyrin PCI-2050 treated A549 human lung cancer cell cultures replicate B |
| GSM147370 |
1.25uM sapphyrin PCI-2050 treated A549 human lung cancer cell cultures replicate C |
| GSM147520 |
2.5uM sapphyrin PCI-2050 treated A549 human lung cancer cell cultures replicate A |
| GSM147521 |
2.5uM sapphyrin PCI-2050 treated A549 human lung cancer cell cultures replicate B |
| GSM147522 |
2.5uM sapphyrin PCI-2050 treated A549 human lung cancer cell cultures replicate C |
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| Relations |
| BioProject |
PRJNA99711 |
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