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| Status |
Public on Jan 08, 2021 |
| Title |
SUMOylation regulates the protein network and chromatin accessibility at glucocorticoid receptor-binding sites [array] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Glucocorticoid receptor (GR) is an essential transcription factor (TF), controlling metabolism, development and immune responses. SUMOylation regulates chromatin occupancy and target gene expression of GR in a locus-selective manner, but the mechanism of regulation has remained elusive. Here, we identify the protein network around chromatin-bound GR by using selective isolation of chromatin-associated proteins and show that the network is affected by receptor SUMOylation, with several nuclear receptor coregulators and chromatin modifiers preferring interaction with SUMOylation-deficient GR and proteins implicated in transcriptional repression preferring interaction with SUMOylation-competent GR. This difference is reflected in our chromatin binding, chromatin accessibility and gene expression data, showing that the SUMOylation-deficient GR is more potent in binding and opening chromatin at glucocorticoid-regulated enhancers and inducing expression of target loci. Blockage of SUMOylation by a SUMO-activating enzyme inhibitor (ML-792) phenocopied to a large extent the consequences of GR SUMOylation deficiency on chromatin binding and target gene expression. Our results thus show that SUMOylation modulates the specificity of GR by regulating its chromatin protein network and accessibility at GR-bound enhancers. We speculate that many other SUMOylated TFs utilize a similar regulatory mechanism.
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| Overall design |
Total RNA isolated from isogenic HEK293 cells stably expressing wild-type GR (HEK293flpGR) treated with non-targeting control (siNON) or UBC9 (siUBC9) siRNA for 72 h and exposed to either vehicle (EtOH) or 100 nM of dexamethasone (dex) for 6 h. All conditions are performed in triplicate.
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| Contributor(s) |
Paakinaho V, Malinen M, Lempiäinen JK, Niskanen EA, Palvimo JJ |
| Citation(s) |
33524141 |
| |
| Submission date |
Dec 19, 2014 |
| Last update date |
Apr 17, 2021 |
| Contact name |
Ville Paakinaho |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Eastern Finland
|
| Department |
School of Medicine
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| Lab |
Biomedicine
|
| Street address |
Yliopistonranta 8
|
| City |
Kuopio |
| ZIP/Postal code |
70210 |
| Country |
Finland |
| |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE64373 |
SUMOylation regulates the protein network and chromatin accessibility at glucocorticoid receptor-binding sites |
|
| Relations |
| BioProject |
PRJNA270855 |
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